Problem

Enzyme X is able to catalyze the breakdown of the toxic redcedar dye to CO2 and water. In...

Enzyme X is able to catalyze the breakdown of the toxic redcedar dye to CO2 and water. In a study of the enzyme you have found that the initial velocities (mM · h−1) are dependent on the initial substrate concentration. The data collected are listed below. Plot the data to determine Vmax and KM. How would a tenfold decrease in the enzyme concentration affect the observed KM?

Substrate conc. Initial velocity (mM)

Initial velocity (mM · h−1)

2

5

4

9

6

15

8

18

10

23

12

30

16

38

20

44

25

50

30

55

40

60

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