You are characterizing a novel enzyme X. You measure the velocity of the reaction with different substrate concentrations and obtain the following data:
Initial substrate concentration (mM) | Vo (μg · h−1) |
3.0 | 10.4 |
5.0 | 14.5 |
10.0 | 22.5 |
30.0 | 33.8 |
90.0 | 40.5 |
180.0 | 42.5 |
(a) Determine Vmax and KM.
(b) If the enzyme concentration were decreased to 10% of the amount used in the experiment described above, would the initial reaction velocities change? If so, how?
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