Problem

Solutions For An Introduction to Genetic Analysis Chapter 11 Problem 23P

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Solution 1

The below table shows the relative levels of β galactosidase activity in cells grown under similar conditions:

Glucose

Lactose

Lactose + Glucose

Wild type

0

100

1

lacI -

1

100

1

lacI s

0

0

0

lacO -

1

100

1

crp -

0

1

1

When the cell is lacI-, it leads to absence of negative control by repressor binding, but still under the positive control of CAP-cAMP (Catabolite activator protein-cAMP) binding.
When the cell is lacIs, the transcription will be blocked under all conditions since the repressor does not bind lactose, but still binds deoxyribonucleic acid (DNA).
When the cell is lacO-, it leads to absence of negative control by repressor binding, but still under the positive control of CAP-cAMP binding.

When the cell is crp-, it leads to absence of positive control by CAP-cAMP binding, but still under the negative control of repressor binding.

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Solutions For Problems in Chapter 11