Assessing the equilibrium concentrations in the malate dehydrogenase reaction
A typical intramitochondrial concentration of malate is 0.22 mM. If the ratio of NAD+ to NADH in mitochondria is 20, and if the malate dehydrogenase reaction is at equilibrium, calculate the concentration of oxaloacetate in the mitochondrion at 20°C. A typical mitochondrion can be thought of as a cylinder 1 µm in diameter and 2 µm in length. Calculate the number of molecules of oxaloacetate in a mitochondrion. In analogy with pH (the negative logarithm of [H+]), what is pOAA?
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