Understanding the energetics of the aconitase reaction
(Integrates with Chapter 3.) Aconitase catalyzes the citric acid cycle reaction:
citrateisocitrate
The standard free energy change, ΔG°’, for this reaction is +6.7 kJ/mol. However, the observed free energy change (ΔG) for this reaction in pig heart mitochondria is +0.8 kJ/mol. What is the ratio of [isocitrate]/[citrate] in these mitochondria? If [isocitrate] = 0.03 mM, what is [citrate]?
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