The dimensions of closed circular DNA molecules
The plasmid pBR322 is a closed circular dsDNA containing 4363 base pairs. What is the length in nm of this DNA (that is, what is its circumference if it were laid out as a perfect circle)? The E. coli K12 chromosome is a closed circular dsDNA of about 4,639,000 base pairs. What would be the circumference of a perfect circle formed from this chromosome? What is the diameter of a dsDNA molecule? Calculate the ratio of the length of the circular plasmid pBR322 to the diameter of the DNA of which it’s made. Do the same for the E. coli chromosome.
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