A small DNA molecule was cleaved with several different restriction nucleases, and the size of each fragment was determined by gel electrophoresis. The following data were obtained.
Enzyme | Fragment Size (kb) |
EcoRI | 1.3,1.3 |
HpaII | 2.6 |
HindIII | 2.6 |
EcoRI + HpaII | 1.3,0.8,0.5 |
EcoRI + HindIII | 0.6,0.7,1.3 |
(a) Is the original molecule linear or circular?
(b) Draw a map of restriction sites, showing distances between sites, that is consistent with the data presented.
(c) How many additional maps are compatible with the data?
(d) What would have to be done to locate the cleavage sites unambiguously with respect to each other?
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