A sample of chromatin was partially digested by the enzyme staphylococcal nuclease. The DNA fragments from this digestion were purified and resolved on a polyacrylamide gel. A set of DNA restriction fragments was used as markers. Distances of migration are given below. From these data, estimate the nucleosome repeat distance in the chromatin.
Marker DNA Fragment | Chromatin DNA Fragment | |
Size (bp) | d (cm) | d (cm) |
94 | 40 | 30.5 |
145 | 34.2 | 19.2 |
263 | 25.2 | 14.4 |
498 | 16.7 | 11.5 |
794 | 11.5 | — |
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