Creating and analyzing a hydropathy plot online
What would the hydropathy plot of a soluble protein look like, compared to those in Figure 9.14? Find out by creating a hydropathy plot at www.expasy.ch. In the search box at the top of the page, type in “bovine pancreatic ribonuclease” and click “Go.” The search engine should yield UniProtKB/Swiss-Prot entry P61823. Scroll to the bottom of the page and click “ProtScale” under Sequence Analysis Tools. On the next page, select the radio button for “Hphob. / Kyte and Doolittle,” then scroll to the bottom of the page, and click “Submit.” On the next page, scroll to the bottom of the page and click “Submit” again. At the bottom of the next page, after a few seconds, you should see a hydropathy plot. How does the plot for ribonuclease compare to those in Figure 9.14? You should see a large positive peak at the left side of the plot. This is the signal sequence portion of the polypeptide.
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