Solving the sequence of an oligopeptide from sequence analysis data
Amino acid analysis of an octapeptide gave the following results:
1 Ala | 1 Arg | 1 Asp | 1 Gly | 3 Ile | 1 Val | 1 NH4+ |
The following facts were observed:
a. Trypsin treatment yielded a pentapeptide and a tripeptide.
b. Chemical reduction of the free-COOH and subsequent acid hydrolysis yielded 2- aminopropanol.
c. Partial acid hydrolysis of the tryptic pentapeptide yielded, among other products, two dipeptides, each of which contained C-terminal isoleucine. One of these dipeptides migrated as an anionic species upon electrophoresis at neutral pH.
d. The tryptic tripeptide was degraded in an Edman sequenator, yielding first A, then B:
What is an amino acid sequence of the octapeptide? Four sequences are possible, but only one suits the authors. Why?
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