Solving the sequence of an oligopeptide from sequence analysis data
Amino acid analysis of an octapeptide revealed the following composition:
2 Arg | 1 Gly | 1 Met | 1 Trp | 1 Tyr | 1 Phe | 1 Lys |
The following facts were observed:
a. Edman degradation gave
b. CNBr treatment yielded a pentapeptide and a tripeptide containing phenylalanine.
c. Chymotrypsin treatment yielded a tetrapeptide containing a C-terminal indole amino acid and two dipeptides.
d. Trypsin treatment yielded a tetrapeptide, a dipeptide, and free Lys and Phe.
e. Clostripain yielded a pentapeptide, a dipeptide, and free Phe.
What is the amino acid sequence of this octapeptide?
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