Enzyme Kinetic Equations
REFLECT AND APPLY Would you use a pH meter to monitor the progress of the reaction described in Question? Why or why not?
Question 1
REFLECT AND APPLY The enzyme lactate dehydrogenase catalyzes the reaction
Pyruvate + NADH 1 H+ → lactate + NAD+
NADH absorbs light at 340 nm in the near-ultraviolet region of the electromagnetic spectrum, but NAD+1 does not. Suggest an experimental method for following the rate of this reaction, assuming that you have available a spectrophotometer capable of measuring light at this wavelength.
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