Problem

Problems refer to material presented in Tools of Biochemistry 2A.Suppose you have two gene...

Problems refer to material presented in Tools of Biochemistry 2A.Suppose you have two genetic variants of a large protein that differ only in that one contains a histidine (side chain pKa = 6.4) when the other has a valine (uncharged side chain).

(a) Which would be better for separation: gel electrophoresis or isoelectric focusing? Why?
(b) What pH would you choose for the separation?

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Solutions For Problems in Chapter 2

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