Note that some of these problems refer to information presented in the Tools of Biochemist...

Note that some of these problems refer to information presented in the Tools of Biochemistry 5A-D.

Given the following peptide

(a) Estimate the net charge at pH 7 and at pH 12. Assume the pKa values given in Table 5.1.

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 (b) How many peptides would result if this peptide were treated with (1) cyanogen bromide, (2) trypsin, and (3) chymotrypsin?

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 (c) Suggest a method for separating the peptides produced by chymotrypsin treatment.