For a study of the release of luteinizing hormone (LH), male and female rats kept in constant light were compared to male and female rats in a regime of 14 h of light and 10 h of darkness. Various dosages of luteinizing releasing factor (LRF) were given: control (saline), 10, 50, 250, and 1250 ng. Levels of LH (in nanograms per milliliter of serum) were measured in blood samples at a later time. Analyze the data given in file LHfemale, LHmale to determine the effects of light regime and LRF on release of LH for both males and females. Use both graphical techniques and more formal analyses.
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