A Modified Standard State for Biochemical Applications
MATHEMATICAL For the hydrolysis of ATP at 25°C (298 K) and pH 7, ATP 1 H2O → ADP + Pi + H+, the standard free energy of hydrolysis (ΔG°') is −30.5 kJ mol−1 (−7.3 kcal mol−1), and the standard enthalpy change (ΔH°') is −20.1 kJ mol−1 (−4.8 kcal mol−1). Calculate the standard entropy change (ΔS°') for the reaction, in both joules and calories. Why is the positive sign of the answer to be expected in view of the nature of the reaction?
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