The peptide hormone vasopressin is used in the regulation of saltwater balance in many vertebrates. Porcine (pig) vasopressin has the sequence
(a) Using the data in Figure 5.6 and Table 5.1, estimate the extinction coefficient e (in units of cm2/mg) for vasopressin, using radiation with λ = 280 nm.
(b) A solution of vasopressin is placed in a 0.5-cm-thick cuvette. Its absorbarrce at 280 nm is found to be 1.3. What is the concentration of vasopressin, in mg/cm3? (See Tools of Biochemistry 6A.)
(c) What fraction of the incident light is passed through the cuvette in (b)? (See Tools of Biochemistry 6A.)
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