Why is SDS-PAGE useful as an analytical tool, but not useful to purify your entire target...

Question

Question

Why is SDS-PAGE useful as an analytical tool, but not useful to purify your entire target enzyme?

Answer 1

Answer #1

Due to certain limit of cmc(critical micelle concentration) formation.

It is more useful for analytical tool for separation of protein molecule for it's gel digestion technique.

But it is less useful for it's prepative purpose for separation of larger amount of protein molecule.

Answer 2

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