Benedict's Reagent: A Test for Reducing
Sugars
Carbohydrates are divided into two groups based on the complexity
of their structure. Simple carbohydrates can form either a
single ring structure (monosaccharides) or a
double ring structure (disaccharides -- formed
when a pair of monosaccharides bond). Simple carbohydrates include
familiar sugars such the monosaccharides glucose (the basic fuel of
cells) and fructose (found in fruits). Common disaccharides include
sucrose (table sugar) and lactose (the sugar in milk).
Complex carbohydrates (polysaccharides) are chains of
many bonded simple carbohydrates, and are often used for
energy storage. These include starch, cellulose, and
glycogen.
One test for the presence of many simple carbohydrates is to use
Benedict's reagent. It turns from turquoise to yellow or orange
when it reacts with reducing sugars. These are simple carbohydrates
with unbound aldehyde or ketone groups. In lab, we used Benedict's
reagent to test for one particular reducing sugar: glucose.
Seliwanoff’s test is a chemical test which distinguishes between aldose and ketose sugars. If the sugar contains a ketone group, it is a ketose. If a sugar contains an aldehyde group, it is an aldose. This test relies on the principle that, when heated, ketoses are more rapidly dehydrated than aldoses
Iodine Test: Iodine test is an indicator for the presence of starch. Iodine solution (iodine dissolved in an aqueous solution of potassium iodide) reacts with starch producing a blue-black color
Tests for Carbohydrates F. Testing Foods for Carbohydrates Food Item Tested Benedict's test O Seliwanoff's test...
Tests for Carbohydrates F. Testing Foods for Carbohydrates Food Item Tested Benedict's test 070 Seliwanoff's test Iodine test Carbohydrates present 20 REPORT SHEET Tests for Carbohydrates Water Glucose Fructove Lactove Sucrose Stardh Takues Test Benedict's Test for Reducing Sugars A. 1. Observations 2. Reducing Nonreducing B. Seliwanoff's Test for Ketoses 1. Colors after 1 min 2. Ketose/Aldose C. Fermentation Test 1. Observations 2. Fermentation D. Iodine Test for Polysaccharides 1. Observationsa 2. Amylose (Yes/No) Questions and Problems 01 From the...
Report Sheet Carbohydrates Results of Carbohydrate Tests D. Benedict's Test E. Seliwano T Glucose e rmentation Test G. Todine Test Fructose Sucrose Lactose Starch Water Questions and Problems 0.6 From the results above, list the sugars that are reducing sugars and those that are not Reducing sugars Nonreducing sugars Q.7 What sugars are ketoses? Q.8 What sugars give a positive fermentation test? Q.9 Which carbohydrates give a blue-black color in the iodine test?
Biuret reagent tests for proteins. There are other reagents that test for other macromolecules. For carbohydrates, Benedict’s reagent tests for simple sugars and iodine (IKI) tests for starch. For lipids, a simple grease spot test using a brown paper bag can be performed, where fatty substances will cause the spot to be translucent when held up to a light. Record the expected colors of solutions of the following substances when tested with these reagents and explain your hypotheses: Bread:...
Questions A-F
(Molecule above is aldotriose)
drate diagnostic tests, the Benedict's Test for "reducing was once the standard Benedict's test is proportional to the amount of "reducing sugar" present 2. Of the carbohyd test for glucose in the urine of diabetics. The amount of brick-red precipitate formed in the sugars" is perhaps the most significant, as it in the urine. tion of the Benedict's test, the two reactants are the sugar and the Cu?' ion. Consider performing the Benedict's Test...
Lab question
Table 5A-1 Data Table for Benedict's and Lugol's Tests Benedict's Test Lugol's Test Origia Final Color Color Before Boiling Tube After After 1. Water 2. Starch 3. Glucose elue owrm 5. Sucrose 6. Onion juiceGen Crenve 7. Potato slice l ecue | Green | WhLte一一1tra-areen 8. Milk Did the results for each test support your hypotheses? Did your results support your null hypothesis? Did the results for cach test agree with your predictions? Explain any discrepancies. PART 2-LUGOL'S...
consider a drug testing company that provides a test for
marijuana usage. among 329 tested subjects, results from 30 were
wrong. Use a 0.05 significance level to test the claim that less
than 10 percent of the results are wrong.
Consider a drug testing company that provides a test for marijuana usage. Among 329 tested subjects, results from 30 subjects were wrong (either a falso positive or a false negative). Use a 0.05 significance level to test the claim that...
Consider a drug testing company that provides a test for marijuara usage. Among 301 tested subjects, results from 27 subjects were wrong (either a faise positive or a faise negative. Une 0.01 significance level to test the claim that less tha 10 percent of the best results are wrong. Identify the null and alterative hypotheses for this test. Choose the correct answer below. O A M:p=0.1 Hp01 OB. Hy:p 0.1 Hyp<0.1 OC. Hyp<0.1 Hp0.1 OD Mp=0.1 Hyp#01 Identity the best...
Consider a drug testing company that provides a test for marijuana usage. Among 250 tested subjects, results from 30 subjects were wrong (either a false positive or a false negative). Use a 0.05 significance level to test the claim that less than 10 percent of the test results are wrong. Identify the null and alternative hypotheses for this test. Choose the correct answer below. O A Ho: p=0.1 H, p<0.1 Ho: p=0.1 IPi1 Hp0.1 Ос. Ho: p<0.1 H, p=0.1 OD....
tests to see whether they are using performance-enhancing drugs. Officials select two teams at up to test all the players of the randomly chosen teams. Each testing day can be considered a study of drug use in baseball. Answer parts a) and b). unan a) What kind of sample is this? O Systematic sample Voluntary response sample Cluster sample Stratified random sample Simple random sample Convenience sample O O O b) Is the choice of sampling design appropriate? A. No....
Holliston Test Laboratories does heat testing (HT) and stress testing (ST) on materials and operates at capacity. Under its current simple costing system, Holliston aggregates all operating costs of $1,275,000 into a single overhead cost pool. Holliston calculates a rate per test-hour of $15 ($1,275,000 / 85,000 total test-hours). HT uses 60,000 test-hours, and ST uses 25,000 test-hours. Gary Sarsfield, Holliston's controller, believes that there is enough variation in test procedures and cost structures to establish separate costing and billing...