How many microliters of original sample are required to produce a final dilution of 10-3 in...
1)How many microliters of original sample are required to produce a final dilution of 10-2 in a total volume of 5 mL? 1 microliter is 10-6 L or 10-3 mL. 2)A 0.00001 dilution is performed on a culture of bacteria in order to perform viable plate counts. From the dilution, *0.1 mL* of solution is plated on solid media, and 197 colony forming units grow on the plate. How many bacteria are in a single mL of the original culture?...
(A) You have a sample with an original concentration of 1.0 x 10^7 CFU/mL. With the Plate Count Method, what final dilution factor would be needed to produce countable plates? Show your work. (B) Describe a dilution scheme (how many tubes, what volume in each tube, what DF is achieved in each step) that uses only the 9-mL blank diluent tubes to achieve the dilution needed for this FDF.
How much serum is present in 50 micrometers if the sample is diluted 1:5? 0.4 microliters 1 microliter 10 microliters 0.2 microliters
3. A serial dilution of a swab sample was prepared by adding 10 uL to a volume of 990 ML water in tube 1, and 10 uL to a volume of 990 uL water in tube 2. a. What is the overall dilution and dilution factor of each tube? Overall Dilution Dilution Factor Tube 1 Tube 2 b. If 100 uL of tube 2 is spread plated and 126 colonies grow, what was the #cells/mL in the original sample?
Dilution is a process in which the concentration of a solution is lowered by adding more solvent. Some of the reasons dilutions are performed are to minimize measurement errors when preparing a series of solutions at different concentrations, to save time and laboratory space, and to make more accurate measurements on an analytical balance when the target concentration is very low. The new concentration of a diluted solution can be determined from the following equation, sometimes called the dilution equation,...
1. You have been given a sample that is a 1/50 dilution of the original. You measure 3.4 mg glucose in 0.5 ml of that sample. What is the concentration in the original sample? If you have 400 ml of that sample, how many grams of glucose is in the total sample?
You add 10 ul of a 1:10 dilution of Sample A to a total volume of 1.21 ml, and obtain a rate of 0.8 Δ A min. What is the corresponding activity (in molimin/ml) in the original sample?
14. Analyze the following serial dilution experiment and answer the questions, each (a and b) worth 10 pts. Given: One ml of the original bacterial culture (tube 0) was added to experiment tube 1, containing 9 ml of media. 7 more experiment tubes were serially diluted in order, the same way, for a total of 8. Each originally contained 9 ml of media, and each received 1 ml from the tube number preceeding it. Two drops from tube #8 were...
6) How many bacteria would be in 1 ml of the original sample if the 108 dilution has 200 colonies? 7) What criteria must your plates meet in order to be considered "countable" ? How many bacteria per milliliter of sample would be "acceptable" to the FDA? - 86 -
A 0.5-L unbuffered solution needs the pH adjusted from 6 to 3. How many microliters of a 2 molar HCl solution need to be added to adjust the pH? Report your answer to four decimal places. For this question: Assume the volume of HCl being added is negligible and the total volume of the solution does not change. The volume of the solution is *not* 1 L like it was in the practice pools. The question requests your answer be...