You add 10 ul of a 1:10 dilution of Sample A to a total volume of...
3. A serial dilution of a swab sample was prepared by adding 10 uL to a volume of 990 ML water in tube 1, and 10 uL to a volume of 990 uL water in tube 2. a. What is the overall dilution and dilution factor of each tube? Overall Dilution Dilution Factor Tube 1 Tube 2 b. If 100 uL of tube 2 is spread plated and 126 colonies grow, what was the #cells/mL in the original sample?
Dilution, help?? 5. You add 25uls of antibody to 475 uls of water - what dilution did you just make? 6. (3 pts) To obtain a countable plate to quantify your bacterial sample you first dilute the sample 1/500 in Tube 1 and then make a 1/25 dilution of this sample in Tube 2. If the total volume for your dilutions is 1 ml: a. How much water should be in Tube 1 and how much of your initial bacterial...
ul of your 1. If you want to make a 1/4 dilution of a sample with the goal of having a final volume of 800 PL, you have to add sample to pl. of your diluent. -> Moving to another question will save this response. Question 1 of 12
1. You want to prepare a 1:50 dilution of your protein extract in a total volume of 1000 uL. You will need ___ uL of protein extract and ___ uL of water. 2. You are provided with a solution of BSA that is 100 ug/mL and make a 10-2 dilution? What is the resulting concentration of the DILUTED BSA? 3. Which of the following statements about enzymes is true? Select one: a. Enzymes increase the rate of a chemical reaction...
5. Complete the following dilution table. NOTICE UNITS OF MEASUREMENT Patient Sample (uL) Diluent (mL) Dilution Factor 10 (1:10 20 "uL 45 uL 50 ul 100 ul 150 uL 0.18 "mL" 0.090 mL 10 C. 0.400 mL 3
After making a serial dilution a tank water sample, you plate 100 ul of the 10-4 After incubating, you count 90 colonies on the plate. What is the CFU/ml? Write out your calculations, briefly, along with your final answer.
How many microliters of original sample are required to produce a final dilution of 10-3 in a total volume of 1,000 mL? • 1 microliter is 10-6 L or 10-3 mL. • The dilution is equal to M2/M1
1. You have been given a sample that is a 1/50 dilution of the original. You measure 3.4 mg glucose in 0.5 ml of that sample. What is the concentration in the original sample? If you have 400 ml of that sample, how many grams of glucose is in the total sample?
You spread 0.1 mL volume of a 10^(-5) dilution onto a nutrient agar plate. After 24 hours of incubation at 37°C, there were 223 colonies of bacteria on the plate. A.) What is the original concentration (OCD) of bacteria in the stock sample this dilution came from? (2 points) B.) Using the OCD value from part A, determine the number of colonies that would be expected to grow on a plate that is inoculated with 0.1 mL volume of 10^(-7)...
A. 0.001 uL B. 0.01 uL C. 0.1 uL D. 1.0 uL You have performed a 10-fold serial dilution of your phage sample from 100 to 10-4. You have been transferring 10 uL of sample from one tube to the next. How many microliters of the original 100 sample are in the 10-4 tube?