Question

This test is used as a screening test for infection with tiv. What might account for false negative results? klhat might acrout for frilse positive results? Diagram this lest Include solid phase Support, patient antibody, conjugate, substrate product indicator Name the substrate enzyme and the for the essay What color is a postive reaction.

Answer 1

1) Human immunodeficiency virus is an etiological factor to cause HIV infection. It is caused by contamination with blood,semen or vaginal fluids from the infected patient mainly through unprotected sexual contact, during transfusion of blood and blood products from HIV infected person,sharing needles, through placenta from mother to baby during the time of labor. Immunoassay test is done to screen the presence of HIV in infected patient. A positive results for HIV infection is not only confirmatory results more test can also be down to make confirmed diagnosis. False Negative results commonly occurs when the infected one is in a Window period, in which there is no adequate antibodies and antigens circulating in the blood.

2) Enzyme linked immunosorbent assay uses enzymes with alkaline phosphatase and three substrates. p-Nitrophenyl phosphate used as a substrate to identify alkaline phosphatase in ELISA. The substrate of the enzyme normally used in ELISA is chromogenic substance.

3) Color changes occurs when blood sample exposed to substrate and when the antibody is detected. There is no change in color if there is antibody detected.