There is a substance in a solution (8g/liter). The length of a cuvette is 1cm and only 25% of the certain light beam is transmitted. What is the absorption coefficient?
We have , Absorbance = log ( I 0 / I )
Where , I 0 intensity of incident light and I intensity of transmitted light.
We have, I 0 =100 % and I = 25 % .
Therefore, A = log (100 /25 ) = 0.602
From Beer's law , we have relation A = b C
Where A is absorbance, is absorption coefficient , b is path length and C is concentration of substance.
0.602 = 1 cm 8 g / L
= 0.602 / 1 cm 8 g / L = 0.075 g L -1 cm -1
ANSWER : absorption coefficient of substance = 0.075 g L -1 cm -1
There is a substance in a solution (8g/liter). The length of a cuvette is 1cm and...
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