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QUESTIONS Consider the controls. a. Are the uninoculated tube and the saline on the slide positive or negative controls? egat
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b) Catalase is an enzyme produced by all living organisms as they exposed to oxygen. Certain pathogenic organisms like streptococcus, enterococcus are catalase negative. The organisms like pseudomonas, candida, aspergillus, staphylococcus are catalase positive. In uninoculated tube test the catalase positive organisms are identified by adding hydrogen peroxide solution. As the H2O2 is added to the medium if the Catalase positive organisms is present it generates bubble within a minute of time as the enzyme Catalase breaks harmful hydrogen peroxide into water and oxygen.

c) Saline solution is used in the coagulase test to differentiate the presence of staphylococcus or streptococci organisms.

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