Question

7. Lysozyme has two key catalytic residues, Glu 35 with a pKa of 5.9 and Asp 52 with a pKa of 4.5. If the pH optimum for this enzyme is 5.0, what does this tell you about the mechanism? a. Glu 35 acts more likely a general acid than Asp 52. b. Glu 35 acts more likely a general base than Asp 52. c. Asp 52 acts more likely a general acid than Glu 35. d. None of the above is correct. 8. In the following serine protease enzyme-substrate reaction 1, which reaction is NOT included?

Answer 1

77 a Glu 35 Glue 35 acts more likely a general acid than Asp 52. if pH > Pka, the proaton is lost (base) pH Leka, u & is intact Calid).