why do we start with the highest dilution (lowest amount of enzyme) when running the enzyme...

Question

Question

why do we start with the highest dilution (lowest amount of enzyme) when running the enzyme reactions

Answer 1

Answer #1

1. It will lose the cofactors that are required for full activity.

2. The protein will not be able to maintain the conformation in dilute solutions without the proper osmoticum.

Answer 2

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