Question

Laboratory 10 Determination of the Concentration of a Protein 145 (b) For Part I, describe how each error listed below would affect the accuracy of calculating the unknown's concentration. Please explain if the R-squared value would help detect each of these errors. Error #1: The concentration of the BSA stock known solution is higher than labeled [4 points) Error #2: The concentration of an individual known sample is higher than labeled. [4 points)

Answer 1

AnsueR (b) (iveu - Discribe ho Each Evor isted be las oud aflers the accova cy of Calculatg the ontuah's onteutretioh Evror 1 The Con centration of the RSA stocle lchasn Solution is higher than labeld let Your stock Solution had a higher Concentration than what it Says in the level. yuu woold be preparing Solutions with haher Concentretious than hat Standa Yd Yau ave thinking meaws that you wilt get a good (alibration This Cuvue in tevus ol its R-squaved alue so, you ont be able to detect the tnwor usiug this lalue, But Yu hould be obtainig Evoneus alues fer your bnknouns. O Since, the slope ef the Catibraetion Covve woud be higher tau lat it should be. Ewov 2: The concentration of an individual kuash is higher than labeted Sanpie

highev Cancentnth f a singe standard has CA than smpected. You ould hotice t outd be ovtside ef te tinear tvend tue vest af the Standards folla, affectvg y indi ative heutd be agood R-Squave d Vatue, Lhich of the Evror