Homework Answers
Solution 1 :
If we increase the enzyme concentration, the rate of reaction will speed up (as long as substrate is available). After the binding up of all the substrates the reaction then doesn't speed up even if we increase the substrate concentration.
Solution 2 :
Lactase functions in acidic environment. Its optimum pH is 6.
Add Answer to:
COST-LAB QUESTIONS: 1. What happens to enzyme activity as the enzyme concentration is increased? 2. Based...
POSTLAB QUESTIONS Name: Masa Ahmad 1. What were the optimal pH, temperature and enzyme concentration for...
POSTLAB QUESTIONS Name: Masa Ahmad 1. What were the optimal pH, temperature and enzyme concentration for catalase? 2. Define the term denatured. Denatured is the Breaking the bonds on the molecules such as proteins 3. Which condition tested in lab denatured the catalase? 4. Why does product production slow down over time, even at optimal pH, temperature and enzyme concentration? 5. Amylase is an enzyme that is produced in human salivary glands and secreted into the mouth to aid in...
Enzymes Temperature is an important factor that can regulate enzyme-mediated functions. Temperature is one of the...
Enzymes Temperature is an important factor that can regulate enzyme-mediated functions. Temperature is one of the tightly homeostatically controlled variables in the human body. What are the symptoms of loss of temperature control during a high fever? Explain why these symptoms occur based on enzyme function and cell signaling effects that are dependent on temperature. Based on Figure 1, explain how the temperature optimum of the activity of enzymes is expected to differ in organisms depending on where they live....
When running enzyme assays, why do you need to normalize enzyme activity to total protein concentration?...
When running enzyme assays, why do you need to normalize enzyme activity to total protein concentration? What does the specific activity mean in the research lab where enzymes are used? (Hint: are enzymes always pure?)
The rate of an enzyme-catalyzed reaction initially increases with an increase in the substrate concentration, but...
The rate of an enzyme-catalyzed reaction initially increases with an increase in the substrate concentration, but eventually reaches a maximum value, even though the concentration of substrate continues to increase. Which of the following best explains why? O As substrate concentration increases, the substrates preferentially bind with each other instead of the active site of the enzyme, and no additional catalysis occurs. As substrate concentration increases, the active sites of all the enzyme molecules become occupied with substrate molecules, and...
Help on prelab NOTE! There are FOUR (4) pages in this pre-lab! PRE-LAB WORKSHEET FOR ENZYME...
Help on prelab
NOTE! There are FOUR (4) pages in this pre-lab! PRE-LAB WORKSHEET FOR ENZYME LAB To be completed prior to the online prelab esercise 1 ) Suppose that the diagram below represents what occurs during a chemical reaction e) Which letter points tq the prodact? print e ore , d 3) (c) Which letter points to the enzyme? (d) Which letter points to the enzyme's active site? (e) Which letter points to the enzyme's substrate? Note: Some letters...
D Each enzyme is specihic of and because its shape catalyzes on certain reactions. The chemical reactions taking place in a cell will most likely speed up if the 3. A genetic material stops re...
D Each enzyme is specihic of and because its shape catalyzes on certain reactions. The chemical reactions taking place in a cell will most likely speed up if the 3. A genetic material stops replicating. B the size of the cell increases. C the enzymes involved become inactive. D the concentration of the reactants is increased. 4. Enzymes A are able to heat up molecules so that they can react B provide CO2 for chemical reactions. C are biological catalysts....
We recently used an enzyme in the laboratory: lactase. I have also often used the metaphor...
We recently used an enzyme in the laboratory: lactase. I have also often used the metaphor "Goldilocksian" to describe the catalysts we call enzymes. Our results in our experiments did not exactly show the traditional relations of enzymes to different levels of pH and temperature. Thus, I provide here a "theoretical" set of data (closer to what would be seen with more ideal conditions). Absorbance at 0° 20° 400 600 7001000 Looking at the theoretical relation of enzyme activity to...
Does anyone know how to do this? Thanks in advance! Enzyme Activity (ml) Effect of pH...
Does anyone know how to do this? Thanks in advance!
Enzyme Activity (ml) Effect of pH on (amylase) enzyme activity. For determination of optimum assay pH of the enzyme reaction, 0.05 M Na2HPO4-NaH2PO4 buffer was used. The reaction was carried out for 10 min at 50°C in a shaking water. The enzyme activity was measured and the results are presented on graph, Bars represent means standard deviations for three replicates. 1. What do the error bars in this graph represent?...
2. hypothesize the best conditions (pH and temperature) under which the enzyme chymotrypsin functions with an...
2. hypothesize the best conditions (pH and temperature) under
which the enzyme chymotrypsin functions with an appropriate
reference. also, hypothesize what will occur in the presence of an
inhibitor and the type of inhibition.
EXPERIMENT 5: ENZYME ACTIVITY WITH a-CHYMOTRYPSIN Prelab Assignment 1. Prepare a flow chart, covering one half of the experimental work (either part A and C if your lab bench is on the window side of the lab or part B and D if your locker is...
question 3: what happens to the structure of a protein when it is denatured? question 4:...
question 3: what happens to the structure of a protein when it is
denatured?
question 4: why is it important for a protein to be at a
certain ph to carry out its function
zymes in your textbook, especially the sections on enzym REPORT: Your report should include two separate files: a table of the data from your enzyme experiments online and conclusions consisting of answers to the questions. WARM-UP QUESTIONS: 1. What part of the name 6-O-a-L-ramnosyl-D-glucosidase shows that...
POSTLAB QUESTIONS Name: Masa Ahmad 1. What were the optimal pH, temperature and enzyme concentration for catalase? 2. Define the term denatured. Denatured is the Breaking the bonds on the molecules such as proteins 3. Which condition tested in lab denatured the catalase? 4. Why does product production slow down over time, even at optimal pH, temperature and enzyme concentration? 5. Amylase is an enzyme that is produced in human salivary glands and secreted into the mouth to aid in...
Enzymes Temperature is an important factor that can regulate enzyme-mediated functions. Temperature is one of the tightly homeostatically controlled variables in the human body. What are the symptoms of loss of temperature control during a high fever? Explain why these symptoms occur based on enzyme function and cell signaling effects that are dependent on temperature. Based on Figure 1, explain how the temperature optimum of the activity of enzymes is expected to differ in organisms depending on where they live....
The rate of an enzyme-catalyzed reaction initially increases with an increase in the substrate concentration, but eventually reaches a maximum value, even though the concentration of substrate continues to increase. Which of the following best explains why? O As substrate concentration increases, the substrates preferentially bind with each other instead of the active site of the enzyme, and no additional catalysis occurs. As substrate concentration increases, the active sites of all the enzyme molecules become occupied with substrate molecules, and...
Help on prelab
NOTE! There are FOUR (4) pages in this pre-lab! PRE-LAB WORKSHEET FOR ENZYME LAB To be completed prior to the online prelab esercise 1 ) Suppose that the diagram below represents what occurs during a chemical reaction e) Which letter points tq the prodact? print e ore , d 3) (c) Which letter points to the enzyme? (d) Which letter points to the enzyme's active site? (e) Which letter points to the enzyme's substrate? Note: Some letters...
D Each enzyme is specihic of and because its shape catalyzes on certain reactions. The chemical reactions taking place in a cell will most likely speed up if the 3. A genetic material stops replicating. B the size of the cell increases. C the enzymes involved become inactive. D the concentration of the reactants is increased. 4. Enzymes A are able to heat up molecules so that they can react B provide CO2 for chemical reactions. C are biological catalysts....
We recently used an enzyme in the laboratory: lactase. I have also often used the metaphor "Goldilocksian" to describe the catalysts we call enzymes. Our results in our experiments did not exactly show the traditional relations of enzymes to different levels of pH and temperature. Thus, I provide here a "theoretical" set of data (closer to what would be seen with more ideal conditions). Absorbance at 0° 20° 400 600 7001000 Looking at the theoretical relation of enzyme activity to...
Does anyone know how to do this? Thanks in advance!
Enzyme Activity (ml) Effect of pH on (amylase) enzyme activity. For determination of optimum assay pH of the enzyme reaction, 0.05 M Na2HPO4-NaH2PO4 buffer was used. The reaction was carried out for 10 min at 50°C in a shaking water. The enzyme activity was measured and the results are presented on graph, Bars represent means standard deviations for three replicates. 1. What do the error bars in this graph represent?...
2. hypothesize the best conditions (pH and temperature) under
which the enzyme chymotrypsin functions with an appropriate
reference. also, hypothesize what will occur in the presence of an
inhibitor and the type of inhibition.
EXPERIMENT 5: ENZYME ACTIVITY WITH a-CHYMOTRYPSIN Prelab Assignment 1. Prepare a flow chart, covering one half of the experimental work (either part A and C if your lab bench is on the window side of the lab or part B and D if your locker is...
question 3: what happens to the structure of a protein when it is
denatured?
question 4: why is it important for a protein to be at a
certain ph to carry out its function
zymes in your textbook, especially the sections on enzym REPORT: Your report should include two separate files: a table of the data from your enzyme experiments online and conclusions consisting of answers to the questions. WARM-UP QUESTIONS: 1. What part of the name 6-O-a-L-ramnosyl-D-glucosidase shows that...
Most questions answered within 3 hours.
-
1.b. Fiscal policy is said to suffer from ‘crowding out’.
Explain what this means and why...
asked 1 minute ago -
The equation for the reaction of nitrogen and oxygen to form
nitrogen oxide is written as...
asked 5 minutes ago -
A scientist reproducing some photoelectric effect experiments
shines a light on a metal electrode, but doesn't...
asked 8 minutes ago -
In a study designed to test the effectiveness of magnets for
treating back pain, 35 patients...
asked 28 minutes ago -
Here are summary statistics for randomly selected weights of
newborn girls:
nequals=193,
x overbarxequals=30.5
hg,
sequals=7.3...
asked 17 minutes ago -
Exercise #3:
Create the “MathTest” class. It will have two class variables:
1) a question and...
asked 20 minutes ago -
In epidemiology, how do you calculate the overall incidence of
cure within two groups? What formula...
asked 24 minutes ago -
A 1 liter solution contains 0.357 M ammonium chloride and 0.268
M ammonia. Addition of 0.295...
asked 25 minutes ago -
What are the advantages and disadvantages of using virtual
reality simulations in health care education?
asked 30 minutes ago -
Given input { 66, 28, 43, 29, 44, 69, 19 } and a hash function
h(x)...
asked 50 minutes ago -
A pebble with mass m is thrown straight up with an initial speed
v0 so that...
asked 54 minutes ago -
Let X be a discrete random variable that follows a
binomial distribution with n = 11...
asked 1 hour ago