Question

POSTLAB QUESTIONS Name: Masa Ahmad 1. What were the optimal pH, temperature and enzyme concentration for catalase? 2. Define the term denatured. Denatured is the Breaking the bonds on the molecules such as proteins 3. Which condition tested in lab denatured the catalase? 4. Why does product production slow down over time, even at optimal pH, temperature and enzyme concentration? 5. Amylase is an enzyme that is produced in human salivary glands and secreted into the mouth to aid in starch digestion. a. What is the substrate in this reaction? b. Where on the enzyme will the substrate bind? c. Based on the fact that this enzyme is found in our bodies, what do you hypothesize would be the: optimal temperature for amylase? optimal pH for amylase? d. What do you think happens to the amylase when it enters the stomach, which has a pH of 22 Unit 7, page 15

Answer 1

1. optimal temperature 37 degree , optimal pH 7 ,the concentration of catalase that had the fastest reaction time was the 75% catalase concentration while the slowest reaction time was the 0% catalase concentration .
2. it means destroy the characteristic properties of (a protein or other biological macromolecule) by heat, acidity, or other effect which disrupts its molecular conformation.
3 . Effects of Temperature, As the temperature increases toward the optimum point, hydrogen bonds loosen, making it easier for catalase to act on hydrogen peroxide molecules. If the temperature increases beyond the optimum point, the enzyme denatures and its structure is disrupted.
4.A common reason for this slowing down of the speed (rate) of the reaction is that the substrate within the mixture is being used up and thus becoming limiting.
5.(a) For amylase the substrate is amylose and amylopectin which are the components of the starch mixture, and for maltase it is the maltose sugar which is the substrate.