Question

in the citric Acio cycle your own words why Draw the irreversible reactions (Including the prep step]. Explain in the control points are important,

Answer 1

total 8 steps will takes place in entire citric acid cycle. and out of 8 only 3 steps ale Isleversible reactions. which are in citrate Synthase si iso citrate de hydrogenase . 11) & -ketoglutelake dehydrogenase } - 3 Control points. (1) Citrate Synthase! Aldot condensation of oxaloacetate with acetyl CoA followed by hy deolyling of COASH to give citrate. Enzyme: Citrate Synthale. followed by hydrolyting of Acetyl-CoA - cool to CASH Elly on & -s- CoA Ho-&- cool cco citraté CH2 2 - citrate Synthase Coo CH2 colo XII coor acetate i iso citrate dehydrogenate: oxidative de carboxylation of iso citrate is Catalyzed by iso citrate de hydrogenase to jam d- keto glutalate. this is and reversible step. is bemored. to NADH NADT is ledured waDt NadHtHt cool <H2 ( glos Iso citrate CO 2 &- ketoglutalate. dehydrogenale Ho Ho -e-H cool

ii) a-keto gluetarate dehydrogenate: 2nd oxidative de Carboxylation Catalyzed by a a-ketogluctarate dehydrogenase. the enzyme isa Complex similar to POH & Co-enzymes TPP, Upoamide & FAD are required. & cor lemoved. coo- COASH Coo | 412 CH2 CH2 CH2 NADH s-CoA., & Succing- CoA. NADT 1.-/keto Toluctalate I coo TIPP Lipounce Page 14 FAD