Question

1-) Silica gel has a bulk density of about 0.3 g/cm3, so 15 g would occupy a volume of 45-50 cm3 (45-50 mL). This quantity is called the:

2-) You are carrying out a chromatographic separation on a 15 mg sample using 6.5 g of silica gel in your column. If you aim for a column height of 10 cm of silica gel, what is the inside diameter of the necessary chromatography column in units of cm?

3-) Consider the compounds 4-tert-butyl-cyclohexanol and 4-tert-butylcyclohexanone. Using silica gel plates ------------------,  will have the higher R_f value when ethyl acetate is used as the developing solvent and ---------------- will have the higher R_f value when dichloromethane (methylene chloride) is the developing solvent.

4-) An ethanol solution of 3.50 mg/100 mL of compound X (150. g/mol) in a 1.00 cm cuvette has an absorbance of 0.972 at the wavelength maximum of 235 nm. Calculate the molar extinction coefficient in units of M^-1 cm^-1.

Answer 1

1) column volume

2)

3) 4 ter butyl cyclohexanol will show higher rf value with ethyl acetate because both are polar and as we know like dissolve like concept polar will be dissolve in pokhar