A 1 to 10 dilution is needed of a patient sample due to the linearity limits...
Homework Answers
As per the question.
1 to 10 dilution means; 1:10
That means out of total 10,
1 part of the sample and 9 parts of diluents are present.
If the total volume of the solution is 100 ul (microliters)
1:10 of 100 ul will be
1/10*100 = 10 ul.sample will be collected from the patient and 90 ul diluents will be added.
After adding 10 ul samples to the 90 ul of diluents the total volume will be 100 ul.
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A 1
to 10 dilution is needed of a patient sample due to the linearity
limits...
1. You have been asked to make a 1:5 dilution of 20mL of patient sample. The...
1. You have been asked to make a 1:5 dilution of 20mL of patient sample. The final volume should be 100mL. Which of the following outlines the correct way to make this dilution? For the other answer options, explain why they are incorrect. A. 10mL of patient sample added to 40mL of saline B. 10mL of patient sample added to 50mL of saline C. 20mL of patient sample added to 100mL of saline D. 20mL of patient sample added to...
If you are making a 1:10 dilution of a 10mg/ml BSA solution using 90ul of water...
If you are making a 1:10 dilution of a 10mg/ml BSA solution using 90ul of water as diluent, how much 10mg/ml of BSA would you add? What would be your final concentration of BSA? If you assayed both these solutions using the Bradford assay which one would have a higher O.D.?
5. Complete the following dilution table. NOTICE UNITS OF MEASUREMENT Patient Sample (uL) Diluent (mL) Dilution...
5. Complete the following dilution table. NOTICE UNITS OF MEASUREMENT Patient Sample (uL) Diluent (mL) Dilution Factor 10 (1:10 20 "uL 45 uL 50 ul 100 ul 150 uL 0.18 "mL" 0.090 mL 10 C. 0.400 mL 3
dilutions dilutions What are the resulting dilutions? Notice units of measurement. a) b) c) 500 L...
dilutions
dilutions
What are the resulting dilutions? Notice units of measurement. a) b) c) 500 L of serum is diluted to 2.0 mL 50 uL of whole blood is pipetted into 0.95 mL of diluent 500 mL of liquid K is added to 4.5 L of water 7. If 10 uL of urine is added to 90 uL of water, and the resulting solution is brought to a final volume of 0.50 mL with water, what is the final dilution...
1) if 5 parts sample is added to 10 parts diluents, what is the dilution that...
1) if 5 parts sample is added to 10 parts diluents, what is the dilution that was just made? 2. What is the ratio if 5 parts sample volume is added to 10 parts diluents volume? 3. In a 1 to 3 dilution, what is the dilution FACTOR? 4. In a 1 to 10 dilution, what is the dilution FACTOR? 5. What is the dilution FACTOR if 40 mcL of sample added to 260 mcL of diluent? 6. What is...
7) If you are making a 1:10 dilution of a 10mg/ml BSA solution using 90ul of...
7) If you are making a 1:10 dilution of a 10mg/ml BSA solution using 90ul of water as diluent, how much 10mg/ml of BSA would you add? 8) What would be your final concentration of BSA? 9) If you assayed both these solutions using the Bradford assay which one would have a higher O.D.? 10) What device would you use to measure O.D.? 11) In your assay you would need a blank. What would you use as a blank? Water,...
7. If complement interferes with test results, the serum sample should be A. treated with an...
7. If complement interferes with test results, the serum sample should be A. treated with an anticoagulant. B. frozen and thawed before testing C. heated to 56°C for 30 minutes. D. centrifuged at 3,000 g for 30 minutes. 8. Calculate the quantities of both diluent and solute needed to prepare 70 ml of a 10% solution of acetic acid. Show your calculations. 9. A new assay is used to test 120 samples from patients with a clinically confirmed diagnosis of...
(A) You have a sample with an original concentration of 1.0 x 10^7 CFU/mL. With the...
(A) You have a sample with an original concentration of 1.0 x 10^7 CFU/mL. With the Plate Count Method, what final dilution factor would be needed to produce countable plates? Show your work. (B) Describe a dilution scheme (how many tubes, what volume in each tube, what DF is achieved in each step) that uses only the 9-mL blank diluent tubes to achieve the dilution needed for this FDF.
How many microliters of original sample are required to produce a final dilution of 10-3 in...
How many microliters of original sample are required to produce a final dilution of 10-3 in a total volume of 1,000 mL? • 1 microliter is 10-6 L or 10-3 mL. • The dilution is equal to M2/M1
1. On a serial dilution with the following tubes set up, 1:2, 1:4, 1:8, 1:16, 1:32,...
1. On a serial dilution with the following tubes set up, 1:2, 1:4, 1:8, 1:16, 1:32, and 1:64, there is no visible agglutination in the 1:64 tube. What is the titer of the antibody? A. 32 B. 16 C. 64 D. Cannot be determined 2. If you want to make 2 mL of a 1:20 dilution, how much serum would be needed? 3. When 1.0 mL of serum is added to 3.0 mL of diluent, what dilution does this represent?...
1. You have been asked to make a 1:5 dilution of 20mL of patient sample. The final volume should be 100mL. Which of the following outlines the correct way to make this dilution? For the other answer options, explain why they are incorrect. A. 10mL of patient sample added to 40mL of saline B. 10mL of patient sample added to 50mL of saline C. 20mL of patient sample added to 100mL of saline D. 20mL of patient sample added to...
5. Complete the following dilution table. NOTICE UNITS OF MEASUREMENT Patient Sample (uL) Diluent (mL) Dilution Factor 10 (1:10 20 "uL 45 uL 50 ul 100 ul 150 uL 0.18 "mL" 0.090 mL 10 C. 0.400 mL 3
dilutions
dilutions
What are the resulting dilutions? Notice units of measurement. a) b) c) 500 L of serum is diluted to 2.0 mL 50 uL of whole blood is pipetted into 0.95 mL of diluent 500 mL of liquid K is added to 4.5 L of water 7. If 10 uL of urine is added to 90 uL of water, and the resulting solution is brought to a final volume of 0.50 mL with water, what is the final dilution...
7) If you are making a 1:10 dilution of a 10mg/ml BSA solution using 90ul of water as diluent, how much 10mg/ml of BSA would you add? 8) What would be your final concentration of BSA? 9) If you assayed both these solutions using the Bradford assay which one would have a higher O.D.? 10) What device would you use to measure O.D.? 11) In your assay you would need a blank. What would you use as a blank? Water,...
7. If complement interferes with test results, the serum sample should be A. treated with an anticoagulant. B. frozen and thawed before testing C. heated to 56°C for 30 minutes. D. centrifuged at 3,000 g for 30 minutes. 8. Calculate the quantities of both diluent and solute needed to prepare 70 ml of a 10% solution of acetic acid. Show your calculations. 9. A new assay is used to test 120 samples from patients with a clinically confirmed diagnosis of...
How many microliters of original sample are required to produce a final dilution of 10-3 in a total volume of 1,000 mL? • 1 microliter is 10-6 L or 10-3 mL. • The dilution is equal to M2/M1
1. On a serial dilution with the following tubes set up, 1:2, 1:4, 1:8, 1:16, 1:32, and 1:64, there is no visible agglutination in the 1:64 tube. What is the titer of the antibody? A. 32 B. 16 C. 64 D. Cannot be determined 2. If you want to make 2 mL of a 1:20 dilution, how much serum would be needed? 3. When 1.0 mL of serum is added to 3.0 mL of diluent, what dilution does this represent?...
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