Question

The last step of glycolysis converts phosphoenolpyruvate to pyruvate. phosphoenolpyruvate + ADP - pyruvate + ATP A G = -33 kJ/mol (-7.5 kcal/mol) The AG® of the reverse reaction is +31 kJ/mol (+7.5 kcal/mol). Instead of reversing the pyruvate kinase reaction, the step is bypassed in gluconeogenesis. Several steps for the conversion of pyruvate to phosphoenolpyruvate via gluconeogenesis are given. Place the steps in the correct order. You will not place all of the steps. First step

Answer 1

Sol Griven data, The last Converts enolpyuvate ascolysts Step of to Pysuwate Pyouvate kinase Phosphoenalpysuwate+ADP Pyouvate ATP 3/mol 5 Ka/ma AG eaction is +31 K3/mo) The A.G of the evesse C+nu5 FCamo from of e Giluconeo genests the n-Casbohy drate Compounds Substrates or guconeogenests ase lactate PTuvate, min adas, propian ate Qnd yceo Synthests The Gluceonengeness the Precusos Only fo Cytosd, b OCcusS mitochondsia Produced of Pvateto in Conver sion In the Mitochondsia and Phosphoenol pyawate Cytosol Step- 1 Psrwate arboxylase a biotin, Tt Converts Pyuvate tO Oxaloacetate and Co Presence of ATP

Oxaloace-taie Tmpeameable, Canet be Sent Out of mtochondsfa So, Its Conver-ted to malate. Step-a inmito chondsi dehy deggenase Malate Synthest3ed in Convests Oxaloacetate malate mito chon dsfal ma-loix-to toCytosol tranparted Malate Stop-3 Malate dehydvogenase esponsrble for Deaction in Cytosol a deversible Oxaloacetate malate to Convests Step-4 Carbory- kinase in Cytosol Phospho enolpysuvate to phospho enolpy ote (Pe) Converts Oxalo acetate hnagh Enegi phosphate oxaloacetate to fsom PEp The Enayme -toansfis band fom GITP to Cos Ond 1besated

Therefose Frrst Step Th he mto chondsion, Pyuvate Casboxylase Corwerts Puvate to Oxaloacetate Malate dehydso genase n -the mtochon dofoo Deduces Oxaloace tate -to malate Malate dehy dogen Cytoplasm in the ase Oid?3es malate to OKaloacetate. Phosphoenolpyuvate Carboxgkinase de carbokylates Oxalo acetate, fomi and Phosphoylates Phosphoenolpyrwate Last Step