Question

(a) In what order do you expect the following species to be eluted in normal-phase LC? Indicate with numbers and provide brief rationale: 0₂N NO₂ NO2 trinitrotoluene 1-heptene heptane heptane phenol toluene (b) Fraction of which component of the mobile phase should be increased when a hexane/acetone gradient is employed to facilitate elution of phenol and trinitrotoluene? Provide brief rationale

Answer 1

Sot No. PT No2 heptane D D NOZ heftene Oh Tohuone 3) (6) trimentoluene 0 de normal ligued Chromal geasly, least polar wal Come out first and most polar will come at the Ind. B ax 342<5<1k4 order of polarity Therefore ③ will come first and most - Polar phenol will come last Hexame is non polar & Accfone is polar, Frost Teinebotoluene will come out first being less polar the phenol. Therefore, we might need to increse percentage of more polar Acetone as we run the columna