Question

A scientist is conducting a Sanger's sequencing experiment to determine the number of polypeptides present in an oligomeric protein. The molecular weight of the protein is 18000 g/mol . After the reaction of 520 mg of the protein with 1‑fluoro‑2,4‑dinitrobenzene, the peptide bonds were hydrolyzed with an acid. As a result, the scientist obtained 39 mg of 2,4‑dinitrophenyl serine. What is the number of the polypeptide chains present in the oligomer?

Answer 1

1-horo-2, 4- dimitro bengene meaet bu'th a forodues alinitro pheny! fatlncing aunin o manner amino aei dl an NUr NH. N* + (ONFB) HF R1 Cydhalai) Nh NM. +HN O of pratain 000 glanel Molas man rotein w Wimker mol of - Bas 2.89 x 10s mol X HN Molas mam ol 2,4, dinim phenyl Sevine 27/'1t/mot 2,4 dinha pheny serine 17

Now anume that theno n m exeept Nrbrmin o na Ane, Ser ine n N-temuno aminoas'd df eae peptide Wumber o Aaf t'ele cham paphia 1 ,4-olinihophony/ Svine Mol of prtein Maln 2 87 X10 Mamsor o yaftide tham frauns