Question

tule U ways one might alter order to improve the the following EXERCISE 15.11: Describe various ways one mio the chromatographic experiment in order to improve resolution of the two, circled peaks seen in the follo chromatogram. Justify your suggestions. 10 20 30 50 60 min HPLC chromatogram of J'Adore perfume (aqueous), an example of a complex mixture analysis. Separation was conducted on a C18 column using a linear 5 - 100% acetonitrile-water gradient over minute run time.

Answer 1

1. By Changing the solvent gradient combination of acetonitrile-water. Eluting for longer time with similar polarity can increase resolution.it can be done by decreasing the % of organic solvent

2. By increasing the plate number or column by increasing the length of the column.

3. Longer column with larger particle packed in the column can increase separation efficiency.