A bacterial culture is diluted 4 times by repeating the same procedure four times (10 ml...
1. You have a bacterial cell culture with a concentration of 1x109 cells/ml. You need to dilute the cells to a concentration of 1x103. What is the correct ratio for this dilution? 2. You add 0.1 ml of a yeast culture to a test tube that contains 9.9 ml of buffer solution. What is the dilution factor of this mixture? 3. In a 1000 µl total dilution volume, the volume of cell culture used is 800 µl. What is the...
You have a bacterial culture solution. When you directly measured the OD600, of the culture solution, it was 3. Then, you performed a 10-fold dilution and measured the OD600 of the diluted solution, which was 0.5. Thirdly, you performed another 10-fold dilution and measured the OD600 of the diluted solution, which was 0.07. Assuming that OD 600 =1 is equivalent to 10^9 cells/ml, how many cells do you have in 1ml of the original culture? You have a bacterial culture...
2) You are given a bacterial culture with the following serial dilutions: 1 ml 0.1 ml 1 ml Original culture 9 ml media 9.9 ml media 9 ml media B a) what is the final dilution in tube C? (1 pt) b) if you plated 100 ul of the bacterial dilution in tube B, incubated and counted 114 colonies the next day, what would the CFU/ml be of the original bacterial culture? (show work and units- 2 pts)
If a solution of 10 mg/ml methyl orange is diluted 100 times, 1 ml added to 99 ml of water for example, what is the final concentration? A. 1 mg/ml B. 0.1 mg/ml C. 0.001 mg/ml
You carry out a serial dilution of a bacterial culture, using dilutions of ⅕, ⅕, 1/100, and 1/10. Then you plate 100 µl of the final dilution and the next day observe 200 colonies on your plate. What was the concentration of the original culture? (5 pts)
A bacterial culture starts at a concentration of 1.0*103 CFU/ml. The generation time is 48 minutes. What is the concentration of the bacterial culture after growing for 16 hours?
c)You have 0.6 mL an undiluted culture at a density of 3.7 x 107 CFU/mL. You add 5.4 mL of sterile diluent. What is the dilution and final cell density? e) Plating 2.0 mL of a sample diluted by a factor or 10-2 procedures 56 colonies. What was the original cell density in the sample? f)Plating 0.2 mL of a sample diluted by a factor or 10-4 procedures 87 colonies. What was the original cell density in the sample?
this is a bacterial growth curve experiment . please explain rhe results Procedure: You will follow the growth of E. coli over the course of the period (3 hrs) by making direct counts of the bacterial suspension by measuring the turbidity of a sample at a given time with a spectrophotometer. The data obtained from the direct counts will be used to plot a partial growth curve. Summary: Turbidity Counts with the Spectrophotometer to measure absorbance at 600. Direct Counts...
The recommended procedure for preparing a very dilute solution is not to weigh out a very small mass or measure a very small volume of stock solution. Instead, it is done by a series of dilutions. A sample of 0.8521 g of KMnO4 was dissolved in water and made up to the volume in a 500.0−mL volumetric flask. A 2.000−mL sample of this solution was transferred to a 1000−mL volumetric flask and diluted to the mark with water. Next, 10.00...
#1-6 1. Calculate the D.F for a bacterial culture if 2000uL of undiluted cuiture was added to a nutrient broth to bring up the final volume to 1 liter. (2 pts) a. 102 b. 2 X 10 c. 2 X 104 2. It you plated 20 ul of sample with a dilution factor of 10-3, what would your total sample volume be? (Total sample volume - (Volume plated) x (Dilution)). (2 pts) 3. Calculate the O.C.D using a viable count...