In preparing a hexane-acetone gradient on a normal phase HPLC column, it is desirable to increase...

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In preparing a hexane-acetone gradient on a normal phase HPLC column, it is desirable to increase...

In preparing a hexane-acetone gradient on a normal phase HPLC column, it is desirable to increase or decrease the proportion of hexane as the column is eluted?

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Answer 1

Answer #1

(normal In HPLC, mobile phase is non-polar (or less polar) than the stationary phase. Therefore, initially the I mobile phase

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