In preparing a hexane-acetone gradient on a normal phase HPLC column, it is desirable to increase...

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In preparing a hexane-acetone gradient on a normal phase HPLC column, it is desirable to increase...

In preparing a hexane-acetone gradient on a normal phase HPLC column, it is desirable to increase or decrease the proportion of hexane as the column is eluted?

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Answer 1

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ANS:This is a liquid solid chromatography where the stationary phase is polar. For such a gradient, one should start with the less polar solvent and increase the percentage of the more polar solvent with time in order to force late eluting solutes (more strongly adsorbed) to leave the column.

[EXTRA POINT - 1. Hexane is non polar and acetone is a polar solvent ]

thank you very much.hope this will help you.good luck

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