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i need help with these 3 problems. Thanks so much
1) How much 600% NaOH should...
Serial dilution problem You have a stock of 5% SDS. You take some of that stock...
Serial dilution problem You have a stock of 5% SDS. You take some of that stock and dilute it 4-fold in tube #1. the tubes hold 1.5ml. give an example of how much 5% stock and how much water you would use to make 4 fold dilution - volume of 5% stock ? - volume of water? - How much volume transferred? - if you took a sample out of tube 2 and did another 4 fold dilution, what concentration...
I just need help with #4. Thank you!! 4. c. PBLAST I have a bacterial concentration...
I just need help with #4. Thank you!!
4. c. PBLAST I have a bacterial concentration of 2500 CRU/mL in tube 'X' in my dilution series. Its total dilution factor (TDF) is 10 What do you estimate the concentration of stock culture (or original tube) to be? *Note: you have all the information you need to perform this calculation (1 pt)
1. You have been asked to make a 1:5 dilution of 20mL of patient sample. The...
1. You have been asked to make a 1:5 dilution of 20mL of patient sample. The final volume should be 100mL. Which of the following outlines the correct way to make this dilution? For the other answer options, explain why they are incorrect. A. 10mL of patient sample added to 40mL of saline B. 10mL of patient sample added to 50mL of saline C. 20mL of patient sample added to 100mL of saline D. 20mL of patient sample added to...
Dilution, help?? 5. You add 25uls of antibody to 475 uls of water - what dilution...
Dilution, help??
5. You add 25uls of antibody to 475 uls of water - what dilution did you just make? 6. (3 pts) To obtain a countable plate to quantify your bacterial sample you first dilute the sample 1/500 in Tube 1 and then make a 1/25 dilution of this sample in Tube 2. If the total volume for your dilutions is 1 ml: a. How much water should be in Tube 1 and how much of your initial bacterial...
1) How would you prepare 500mL of working TBE buffer if the stock solution was purchased...
1) How would you prepare 500mL of working TBE buffer if the stock solution was purchased as 25X TBE? 2) 2mL of a serum sample was added to 18mL of phosphate buffered saline (PBS) in Tube 1. 10mL of Tube 1 was added to 40mL of PBS in Tube What is the dilution of serum in Tube 2? 3) A student needed to prepare 500mL of 1X TAE buffer to run a QC gel. The stock solution in the lab...
1. You have a bacterial cell culture with a concentration of 1x109 cells/ml. You need to...
1. You have a bacterial cell culture with a concentration of 1x109 cells/ml. You need to dilute the cells to a concentration of 1x103. What is the correct ratio for this dilution? 2. You add 0.1 ml of a yeast culture to a test tube that contains 9.9 ml of buffer solution. What is the dilution factor of this mixture? 3. In a 1000 µl total dilution volume, the volume of cell culture used is 800 µl. What is the...
You have a 1 M solution of NaCl. How much do you need to dilute to...
You have a 1 M solution of NaCl. How much do you need to dilute to make 10 L of isotonic saline (0.9%)? Remember that the units of volume and concentration must be the same on both sides of the equation.
1a. I need ___________mLs? 1b. What syringe syringe/syringe size should I select? _________________ 1c. What is...
1a. I need ___________mLs?
1b. What syringe syringe/syringe size should I select?
_________________
1c. What is route of administration? _________________
1d. What is the rate of admnistration? _______________
1e. Should I dilute?_______________. If so, how many mLs of
NS?________________. Will I need to transfer into a larger syringe
if dilutent is needed?__________________If so, what syringe size?
__________________
2. Medication Order: Humulin R (Regular insulin) 6 units
subcutaneously now
Medication dispensed: Humulin R (Regular Insulin) 10,000 units/10mL
(100units/mL) vial
2a. I...
Can someone help me rewrite procedure #3 in paragraph notation. It should be written so that...
Can someone help me rewrite procedure #3 in paragraph
notation. It should be written so that anyone reading it could
replicate what you did.
1. Place cell growth media in 37degree incubator for at least 30 minutes. 2. Remove tubes containing frozen cells from liquid nitrogen tanks and quickly place the tubes on dry ice. Cells should thaw in about a minute. 3. Once thawed, transfer the cells into a 50ml conical tube and add 50ml of warm growth media...
Calculate the initial concentration of Fe3+ in test tubes 1-4. (you will need to take the...
Calculate the initial concentration of Fe3+ in test tubes 1-4. (you will need to take the dilution in to account) Calculate the initial concentration of SCN-in each of test tubes 1-4. (you will need to take the dilution in to account) 2. Label four 20 x 150 mm test tubes 1-4. Pour about 30 mL of 0.0020 M Fe(NO3)3 into a clean, dry 100-ml beaker. Pipet 5.0 mL of this solution into each of the four labeled test tubes. Use...
I just need help with #4. Thank you!!
4. c. PBLAST I have a bacterial concentration of 2500 CRU/mL in tube 'X' in my dilution series. Its total dilution factor (TDF) is 10 What do you estimate the concentration of stock culture (or original tube) to be? *Note: you have all the information you need to perform this calculation (1 pt)
1. You have been asked to make a 1:5 dilution of 20mL of patient sample. The final volume should be 100mL. Which of the following outlines the correct way to make this dilution? For the other answer options, explain why they are incorrect. A. 10mL of patient sample added to 40mL of saline B. 10mL of patient sample added to 50mL of saline C. 20mL of patient sample added to 100mL of saline D. 20mL of patient sample added to...
Dilution, help??
5. You add 25uls of antibody to 475 uls of water - what dilution did you just make? 6. (3 pts) To obtain a countable plate to quantify your bacterial sample you first dilute the sample 1/500 in Tube 1 and then make a 1/25 dilution of this sample in Tube 2. If the total volume for your dilutions is 1 ml: a. How much water should be in Tube 1 and how much of your initial bacterial...
You have a 1 M solution of NaCl. How much do you need to dilute to make 10 L of isotonic saline (0.9%)? Remember that the units of volume and concentration must be the same on both sides of the equation.
1a. I need ___________mLs?
1b. What syringe syringe/syringe size should I select?
_________________
1c. What is route of administration? _________________
1d. What is the rate of admnistration? _______________
1e. Should I dilute?_______________. If so, how many mLs of
NS?________________. Will I need to transfer into a larger syringe
if dilutent is needed?__________________If so, what syringe size?
__________________
2. Medication Order: Humulin R (Regular insulin) 6 units
subcutaneously now
Medication dispensed: Humulin R (Regular Insulin) 10,000 units/10mL
(100units/mL) vial
2a. I...
Can someone help me rewrite procedure #3 in paragraph
notation. It should be written so that anyone reading it could
replicate what you did.
1. Place cell growth media in 37degree incubator for at least 30 minutes. 2. Remove tubes containing frozen cells from liquid nitrogen tanks and quickly place the tubes on dry ice. Cells should thaw in about a minute. 3. Once thawed, transfer the cells into a 50ml conical tube and add 50ml of warm growth media...
Calculate the initial concentration of Fe3+ in test tubes 1-4. (you will need to take the dilution in to account) Calculate the initial concentration of SCN-in each of test tubes 1-4. (you will need to take the dilution in to account) 2. Label four 20 x 150 mm test tubes 1-4. Pour about 30 mL of 0.0020 M Fe(NO3)3 into a clean, dry 100-ml beaker. Pipet 5.0 mL of this solution into each of the four labeled test tubes. Use...
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