What does the Decolorizing Solution, Ethanol, do to Gram-positive bacteria?
Turns it pink/red |
||
Turns it purple/violet |
||
Decolorization |
||
Nothing, because of the thick cell walls |
Decolorization.Ethanol interacts with lipid membrane of both gram positive and gram negative bacteria.Ethanol dehydrates the peptidoglycon layer afterthat which layer is shrinking and tightening.
Large crystal violet does not able to penetrate the tightened peptidoglycon layer.and is thus trapped in the cell in gram positive bacteria.
What does the Decolorizing Solution, Ethanol, do to Gram-positive bacteria? Turns it pink/red Turns it purple/violet...
Steps of Gram Staining Bacteria containing thick cell wall Violet color Bacteria containing thin cell wall (LPS) Violet color Crystal violet treatment lodine Purple color Purple color Decolorization Purple color colorless Safranin Purple color pink 3. A fellow student showed you a gram stained slide where cells containing thick cell walls were stained pink. What would you tell her about the staining procedure? Why? 4. A fellow student showed you a gram stained slide where cells containing LPS were stained...
NEED HELP WITH ALL PLEASE. MICROBIOLOGY You have stained a bacteria using the Gram staining technique what color would any endospores be if present? A. Colorless B. Green C. Pink D. Blue E. Purple 2. If you omitted the Safranin step in a gram staining procedure, what would be the color of a Gram-negative bacteria? A. Colorless B. Pink C. Green D. Purple E. Blue 3. Bacillus licheniformis would be what color and shape after a Gram staining? A. Purple...
QUESTION 15 Why is crystal violet used in the Gram stain retained in the cell wall of Gram positive bacteria, but not in Gram negative bacteria? Gram positive bacteria have a thick peptidoglycan layer while Gram negative bacteria have a thin peptidoglycan layer. Gram positive bacteria form spores and Gram negative do not. O Gram positive bacteria have no peptidoglycan layer and Gram negative bacteria have a thick cell wall O Gram positive bacteria have capsules and Gram negative bacteria...
Gram-positive and gram-negative bacteria differ in that A. Gram-positive bacteria have more peptidoglycan in their cell walls. B. Gram-positive bacteria have fimbriae but gram-negative bacteria do not. C. Gram-positive bacteria are spiral-shaped, and gram-negative bacteria are either rod-shaped or spherical. D. Only gram-negative bacteria can form endospores. E. There is no difference between gram negative and gram positive bacteria F. Gram positive are positively charged and gram negative are negatively charges An unknown bacterial species was found in the blood...
peptidoglycan in their cell wals than Gram (4) bacteria do and thus hold the first of four Gran stains and appear as the color Gram (*) bacteria have microscope more light red loss purple more; purple less light red
1. Please describe the gram staining procedure. Be sure to include: crystal violet, iodine, alcohol, safranin, and any other terms you think are important to the technique. You do not need to re- write the lab procedure here please describe what is occurring conceptually to both gram + and gram-cells during the procedure. What would a gram positive cell look like under oil immersion if a researcher forgot to use crystal violet? Please provide a rationale for your response. 3....
How do acid fast stain results compare to gram stain results? a. In other words, what does the following represent? Acid Fast Stain (to Pink bacteria ACO fas广 Blue bacteria C AS Gram Stain Pink bacteria 6eAm- Violet bacteria GRAM f b. Why is there a difference in what the colors mean?
QUESTIONS Predict the effect on Gram-positive and Gram-negative cells of the following mistakes made when performing a Grum stain. Consider cac mistake independently a. Failure to add the iodine. b. Failure to apply the decoloriser. 3 c. Failure to apply the safranin. d. Reversal of crystal violet and safranin stains. Both crystal violet and safranin are basic stains and may be used to do simple stairs on Gram-positive and Gram-negative cells. This being the case, explain how they end up...
what will happen to a Gram positive cell that you’re Gram staining(usually purple? if you forgot to heat fix it?
- Which of the following pairs is mismatched? d. iodine mordant e. none of the above a. alcohol-decolorizer b. crystal violet-basic dye c. safranin-acid dye 2, Suppose you Gram stained a sample from one colony and observed a field of red and blue cocci. Adjacent cells were not always the same color. You could conclude that you have d. old cells e. made a mistake a. a mixed culture b. young cells c. dividing cells 3. Which of the following...