what will happen to a Gram positive cell that you’re Gram staining(usually purple? if you forgot...
Homework Answers
Heat fixing is done to ensure the adherence of bacteria to the slide so that it could be easily demonstrated if you forget to heat fix the bacteria it would be difficult to identify them
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what will happen to a Gram positive cell that you’re Gram
staining(usually purple? if you forgot...
Steps of Gram Staining Bacteria containing thick cell wall Violet color Bacteria containing thin cell wall...
Steps of Gram Staining Bacteria containing thick cell wall Violet color Bacteria containing thin cell wall (LPS) Violet color Crystal violet treatment lodine Purple color Purple color Decolorization Purple color colorless Safranin Purple color pink 3. A fellow student showed you a gram stained slide where cells containing thick cell walls were stained pink. What would you tell her about the staining procedure? Why? 4. A fellow student showed you a gram stained slide where cells containing LPS were stained...
1. Please describe the gram staining procedure. Be sure to include: crystal violet, iodine, alcohol, safranin,...
1. Please describe the gram staining procedure. Be sure to include: crystal violet, iodine, alcohol, safranin, and any other terms you think are important to the technique. You do not need to re- write the lab procedure here please describe what is occurring conceptually to both gram + and gram-cells during the procedure. What would a gram positive cell look like under oil immersion if a researcher forgot to use crystal violet? Please provide a rationale for your response. 3....
0/1 pts Incorrect Question 8 You have a mixture of gram positive and gram negative cells...
0/1 pts Incorrect Question 8 You have a mixture of gram positive and gram negative cells in culture. You prepare a slide from the culture and gram stain it, but when observing the sample using oil immersion, all cells appear purple. What is the likely technical reason why only purple- colored cells are observ ed? you forgot the mordant step O you killed the cells during heat fixation you forgot the decolorization step you forgot to add immersion oi
NEED HELP WITH ALL PLEASE. MICROBIOLOGY You have stained a bacteria using the Gram staining technique...
NEED HELP WITH ALL PLEASE. MICROBIOLOGY
You have stained a bacteria using the Gram staining technique what color would any endospores be if present? A. Colorless B. Green C. Pink D. Blue E. Purple 2. If you omitted the Safranin step in a gram staining procedure, what would be the color of a Gram-negative bacteria? A. Colorless B. Pink C. Green D. Purple E. Blue 3. Bacillus licheniformis would be what color and shape after a Gram staining? A. Purple...
Exercise 8 - Gram Staining Procedure for gram staining. Know all chemicals/reagents used in correct order,...
Exercise 8 - Gram Staining Procedure for gram staining. Know all chemicals/reagents used in correct order, function of each chemical, the effect of cach chemical and how gram + and gram - bacteria would appear at each step Color reactions at each step of procedure. Know why, based on the differences in cell wall structure, that the gram + vs gram - bacteria stain either purple or pink. What washes off in the decolorizing step of a gram - bacteria?...
SWAU u sulide of the slide Questions 1. What color are Gram-positive cells supposed to be...
SWAU u sulide of the slide Questions 1. What color are Gram-positive cells supposed to be after a Gram-stain? What about Gram-negative cells? (2 pts.) a rum + - purple Gram -- pink 2. What characteristics can be determined in a Gram-stain? (2 pts) 3. List all of the things that can go happen during the Gram-staining process that could lead to an incorrect or poor result. (2pts.) 4. Why are direct gram stains ordered on clinical specimens? (2pts.) 5....
Pre-Lab Questions: After staining what color will Gram positive bacteria be? What color will Gram negative...
Pre-Lab Questions: After staining what color will Gram positive bacteria be? What color will Gram negative bacteria be? What structure is more prominent in Gram positive bacteria than Gram negative bacteria? Why should I not spray water aggressively or directly on my bacteria on my slide?
Gram Staining Safranin O lodine | Crystal Violet | Decolorizer Using the reagents above and the...
Gram Staining Safranin O lodine | Crystal Violet | Decolorizer Using the reagents above and the cells below. Show the steps in Gram staining by drawing arrows and reagents in their proper places. I will start the first step for you. Show the end result of Gram Staining for G+ and G cells. A. Gram Positive B. Gram Negative Step 1: Add Crystal Violet Step 1: Add Crystal Violet Step 2: Add_ Step 2: Add (make sure you show what...
1. Describe several advantages of differential staining procedures compared with simple staining techniques. 2. Give the...
1. Describe several advantages of differential staining procedures compared with simple staining techniques. 2. Give the purpose of each of the following reagents in a differential staining procedure: a. Primary stain b. Counter stain c. Decolorizing agent d. Mordant 3. Why is it important for the counter stain to be a lighter color than the primary stain? 4. You have done gram staining for a gram positive bacteria, but at the end you observed pinkish-red bacteria under microscope, what was...
Your friend performed gram staining procedure on his bacterial sample, but he forgot to wash his...
Your friend performed gram staining procedure on his bacterial sample, but he forgot to wash his slide with 95% alcohol solution. What result would you expect for his sample and is it reliable? explain B 1 ol Certain diseases such as ALS affects motor neurons (neurons that voluntarily sends impulses) rendering them weak and gradually destroying these neurons. Patients having such diseases suffer from difficulty in walking, speaking, holding up posture, breathing, swallowing, and other symptoms. Explain why they would...
Steps of Gram Staining Bacteria containing thick cell wall Violet color Bacteria containing thin cell wall (LPS) Violet color Crystal violet treatment lodine Purple color Purple color Decolorization Purple color colorless Safranin Purple color pink 3. A fellow student showed you a gram stained slide where cells containing thick cell walls were stained pink. What would you tell her about the staining procedure? Why? 4. A fellow student showed you a gram stained slide where cells containing LPS were stained...
1. Please describe the gram staining procedure. Be sure to include: crystal violet, iodine, alcohol, safranin, and any other terms you think are important to the technique. You do not need to re- write the lab procedure here please describe what is occurring conceptually to both gram + and gram-cells during the procedure. What would a gram positive cell look like under oil immersion if a researcher forgot to use crystal violet? Please provide a rationale for your response. 3....
0/1 pts Incorrect Question 8 You have a mixture of gram positive and gram negative cells in culture. You prepare a slide from the culture and gram stain it, but when observing the sample using oil immersion, all cells appear purple. What is the likely technical reason why only purple- colored cells are observ ed? you forgot the mordant step O you killed the cells during heat fixation you forgot the decolorization step you forgot to add immersion oi
NEED HELP WITH ALL PLEASE. MICROBIOLOGY
You have stained a bacteria using the Gram staining technique what color would any endospores be if present? A. Colorless B. Green C. Pink D. Blue E. Purple 2. If you omitted the Safranin step in a gram staining procedure, what would be the color of a Gram-negative bacteria? A. Colorless B. Pink C. Green D. Purple E. Blue 3. Bacillus licheniformis would be what color and shape after a Gram staining? A. Purple...
Exercise 8 - Gram Staining Procedure for gram staining. Know all chemicals/reagents used in correct order, function of each chemical, the effect of cach chemical and how gram + and gram - bacteria would appear at each step Color reactions at each step of procedure. Know why, based on the differences in cell wall structure, that the gram + vs gram - bacteria stain either purple or pink. What washes off in the decolorizing step of a gram - bacteria?...
SWAU u sulide of the slide Questions 1. What color are Gram-positive cells supposed to be after a Gram-stain? What about Gram-negative cells? (2 pts.) a rum + - purple Gram -- pink 2. What characteristics can be determined in a Gram-stain? (2 pts) 3. List all of the things that can go happen during the Gram-staining process that could lead to an incorrect or poor result. (2pts.) 4. Why are direct gram stains ordered on clinical specimens? (2pts.) 5....
Gram Staining Safranin O lodine | Crystal Violet | Decolorizer Using the reagents above and the cells below. Show the steps in Gram staining by drawing arrows and reagents in their proper places. I will start the first step for you. Show the end result of Gram Staining for G+ and G cells. A. Gram Positive B. Gram Negative Step 1: Add Crystal Violet Step 1: Add Crystal Violet Step 2: Add_ Step 2: Add (make sure you show what...
1. Describe several advantages of differential staining procedures compared with simple staining techniques. 2. Give the purpose of each of the following reagents in a differential staining procedure: a. Primary stain b. Counter stain c. Decolorizing agent d. Mordant 3. Why is it important for the counter stain to be a lighter color than the primary stain? 4. You have done gram staining for a gram positive bacteria, but at the end you observed pinkish-red bacteria under microscope, what was...
Your friend performed gram staining procedure on his bacterial sample, but he forgot to wash his slide with 95% alcohol solution. What result would you expect for his sample and is it reliable? explain B 1 ol Certain diseases such as ALS affects motor neurons (neurons that voluntarily sends impulses) rendering them weak and gradually destroying these neurons. Patients having such diseases suffer from difficulty in walking, speaking, holding up posture, breathing, swallowing, and other symptoms. Explain why they would...
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