UV-Vis spectrophotometer is the common detector used for HPLC. If your analytes are aromatic compounds, which absorbance wavelength is often selected for the UV-Vis detector in HPLC?
The wavelength selected for the analytes with aromatic compounds is 280nm, this for the total phenolic determination due to the conjugation of double bonds in the aromatic ring (absorption maximum).
UV-Vis spectrophotometer is the common detector used for HPLC. If your analytes are aromatic compounds, which...
question: double beam UV - VIS spectrophotometer offer the advantge (s) of:- ( multiple choice question ) a) compensate for short term fluctuation in the radiant source and drifts in transducers b) compensate for variations in source intensity with wavelength c) enables continuos recording of absorbance of sample without the blank d) all of the above are correct
An UV-Vis spectrophotometer communicates with a computer using digital signal transmission. The number 100 is used to represent an absorbance of 100 mili absorbance units (mAU). Assume it takes 1.00 ns to transmit a single bit of data. (a) How long does it take to transmit the number 100 in serial form? (b) How long does it take to transmit the number 100 in parallel form using 8 wires? (c) Give one advantage and one disadvantage of parallel digital signal...
which of the 5 types of detectors for HPLC are universal detectors? -UV/Vis -Refractive index -electrochemical -fluorescence -mass spectrometer
A UV-VIS detector is used for the liquid chromatography section of LCMS. q. What B Group vitamins would not show up? (not be detcted, or thought not to be detected)
Which of the following compounds would show the Imax of longest wavelength in its UV-Vis spectrum?
A, What does the UV-Vis spectrophotometer measure? What is Beer’s Law (you might have to look it up) and how does it relate to data collected from a spectrophotometer? B, What is a sample blank? What it is it used for in this experiment? C, What does the addition of NH3(aq) do to the copper(II) solution? If you don’t know because you haven’t yet done the experiment, make an educated guess. (Think about what the NH3 does chemically and what...
8. Which of the following compounds will absorb energy in the region UV-Vis of the magnetic spectrum? B O E None of the above 9. What will be the product of the following reaction sequence? Nanti a Ho.h B C OH D E none of the above
Which of these compounds exhibits the longest wavelength Imax in the UV-vis spectrum? O A. OB. OC. What is the major product of this reaction? 1 equiv. HBr -80°C Br لد A. Br B. OC. Br Br OD. Rank these conformations from least to most stable (less stable < more stable). H Cl CI CH3 H CH3 Н H CH3 CH CI H CH 3 H CH 3 3 H H H - 11 A. II < III < B....
5 A calibration curve used in UV/VIS spectroscopy plots concentration on the y-axis and absorbance on the x-axis. O A) True OB) False Question 5 (2 points) A working standard is prepared by taking 38.00 mL of a 500.0 ppm stock solution and diluting it tos a final volume of 650.0 mL. Calculate the ppm concentration of the working solution. Provide your answer to two places after the decimal and without units. Your Answer: Answer Submit Out 2afactione ad
Table 1. Concentrations of [Fe(Phen),1?, M, in water and UV- vis absorbance values recorded at 2 -505 nm. Concentration of [Fe(Phen)/, M Absorbance 9.500 x 10 0.999 4.250 x 10 0.480 0.219 2.125 10% 1.063 x 10 0.104 0.000 0.003 Table 2. UV-vis absorbance values of [Fe(phen)]* held at 35 °C after mixing with H SO, and recorded at 5 minute intervals (in water at max = 505 nm). Time (minutes) Absorbance 0.997 0.935 0.877 0.823 0.772 0.724 0.680 0.638...