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UV-Vis spectrophotometer is the common detector used for HPLC. If your analytes are aromatic compounds, which...

UV-Vis spectrophotometer is the common detector used for HPLC. If your analytes are aromatic compounds, which absorbance wavelength is often selected for the UV-Vis detector in HPLC?

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Answer #1

The wavelength selected for the analytes with aromatic compounds is 280nm, this for the total phenolic determination due to the conjugation of double bonds in the aromatic ring (absorption maximum).

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