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A calibration curve used in UV/VIS spectroscopy plots concentration on the y-axis and absorbance on...
Question 4 (2 points) A working standard 1 is prepared by taking 24.00 mL of a...
Question 4 (2 points) A working standard 1 is prepared by taking 24.00 mL of a 500.0 ppm stock solution and diluting it to a final volume of 360.0 mL. Working standard 2 is prepared by taking 3.00 mL of working standard 1 and diluting it to a final volume of 250.0 mL. Calculate the ppm concentration of the working standard 2. Provide your answer to three places after the decimal and without units. Your Answer: Answer Question 5 (2...
A set of samples of known concentration were used to create a calibration curve for rubxanthin. The data are shown below. Calibration Curve for Rubixanthin 1.60 1.40 a 1.20 1.00 0.80 0.60 0.40 0.20 0...
A set of samples of known concentration were used to create a calibration curve for rubxanthin. The data are shown below. Calibration Curve for Rubixanthin 1.60 1.40 a 1.20 1.00 0.80 0.60 0.40 0.20 0.00 y 114800x R2 0.9999 0.00E+00 2.00E-06 4.0OE-06 6.00E-06 8.00E-06 1.00E-05 1.20E-05 1.40E-05 Concentration (M) 30) An extract of rubixanthin of unknown concentration was analyzed with UV-Vis Spectroscopy. The unknown had an absorbance of 0.83. What is the concentration (M) of rubixanthin in the unknown?
A...
(10 pts) The iron content of a well water sample is determined by UV-Vis spectroscopy. A...
(10 pts) The iron content of a well water sample is determined by UV-Vis spectroscopy. A 10.00 mL aliquot of well water is transferred to a 100.0-mL volumetric flask and 20 mL of 0.10 M nitric acid is added to oxidize iron (II) to iron (II). Next 30 mL of a 0.1 M sodium thiocyanate solution is added to form the deep red complex iron (II) thiocyanate. The solution is diluted to volume with DI water. A reagent blank is...
Part 2. UV-Visible Spectroscopy Cut2 concentration Absorbance (mol/L) 0.0010 0.053 0.0025 0.128 0.0050 0.251 0.0075 0.378...
Part 2. UV-Visible Spectroscopy Cut2 concentration Absorbance (mol/L) 0.0010 0.053 0.0025 0.128 0.0050 0.251 0.0075 0.378 0.0100 0.510 Diluted unknown, Trial 1 0.079 For your lab notebook: C. Sketch how you could make each of the the 5 standards for Part 2 from a 0.100 mol/L Cu2+ stock solution, 1.25 mol/L NH3 solution, and Dl water. The procedure is referenced in step 2b. Assume you would use 10-mL volumetric flasks to make the solutions. d. Sketch also how you would...
Table 1. Concentrations of [Fe(Phen),1?, M, in water and UV- vis absorbance values recorded at 2...
Table 1. Concentrations of [Fe(Phen),1?, M, in water and UV- vis absorbance values recorded at 2 -505 nm. Concentration of [Fe(Phen)/, M Absorbance 9.500 x 10 0.999 4.250 x 10 0.480 0.219 2.125 10% 1.063 x 10 0.104 0.000 0.003 Table 2. UV-vis absorbance values of [Fe(phen)]* held at 35 °C after mixing with H SO, and recorded at 5 minute intervals (in water at max = 505 nm). Time (minutes) Absorbance 0.997 0.935 0.877 0.823 0.772 0.724 0.680 0.638...
You begin preparation of the calibration curve to measure absorbance vs concentration of FeSCN2+. To do...
You begin preparation of the calibration curve to measure absorbance vs concentration of FeSCN2+. To do so, you add 4.614 mL of 0.200 M Fe(NO3)3 to a cuvette and then directly add 108 µL of 0.001 M KSCN. What is the resulting concentration of FeSCN2+, assuming complete conversion of SCN- to FeSCN2+? Enter your answer in units of mM to four digits after the decimal.
You begin preparation of the calibration curve to measure absorbance vs concentration of FeSCN2+. To do...
You begin preparation of the calibration curve to measure absorbance vs concentration of FeSCN2+. To do so, you add 4.614 mL of 0.200 M Fe(NO3)3 to a cuvette and then directly add 108 µL of 0.001 M KSCN. What is the resulting concentration of FeSCN2+, assuming complete conversion of SCN- to FeSCN2+? Enter your answer in units of mM to four digits after the decimal.
Beer’s Law Objective : We will explore an application of absorption spectroscopy using calibration curves and...
Beer’s Law Objective : We will explore an application of absorption spectroscopy using calibration curves and Beer’s Law. Use the “LAB : HOW TO…” link from the class website if you need help with how to use balance, Bunsen burner… and such. Introduction: You may write this information in your lab notebook for your own reference. It can’t be cut and pasted. Different solutions have different spectral properties. In this portion of the experiment those properties will be utilized to...
A student prepares a caffeine standard solution that has a concentration of 1858 ppm. To prepare...
A student prepares a caffeine standard solution that has a concentration of 1858 ppm. To prepare a calibration curve, the student pipets various volumes of the standard solution into 50.0 mL volumetric flasks and dilutes to the fill line with water. The absorbance of each solution is measured in a 1.00 cm cuvette using the spectrophotometer and is shown in the table below. Calculate the concentration of each solution and create a calibration curve using the absorbance data provided. Based...
Plot of absorbance vs concentration for calibration curve. Data sheet stapled to back of DRA. Solution...
Plot of absorbance vs concentration for calibration curve. Data sheet stapled to back of DRA. Solution Stock solution Dilution 1 (50.0%) Dilution 2 (25.0%) Dilution 3 (12.5%) (SCN) (FeSCNI. 2.0 x10M 2.0 X10M 1.0 x 10-4 M 1.0104M 5.0 x10-5M 5.0*10-5M 2.5 x 10-5Ma.5 x10 -5M 0.836 0.389 0.180 0.085 Calibration curve trendline equation_=43 15.1 % - 0.032 Rvalue of trendline 0.9993 Absorbance Sample Number 0.0020 M Fe(NO), (ml) 0.0020 M KSCN (ML) - - - - - 0. 129...
Question 4 (2 points) A working standard 1 is prepared by taking 24.00 mL of a 500.0 ppm stock solution and diluting it to a final volume of 360.0 mL. Working standard 2 is prepared by taking 3.00 mL of working standard 1 and diluting it to a final volume of 250.0 mL. Calculate the ppm concentration of the working standard 2. Provide your answer to three places after the decimal and without units. Your Answer: Answer Question 5 (2...
A set of samples of known concentration were used to create a calibration curve for rubxanthin. The data are shown below. Calibration Curve for Rubixanthin 1.60 1.40 a 1.20 1.00 0.80 0.60 0.40 0.20 0.00 y 114800x R2 0.9999 0.00E+00 2.00E-06 4.0OE-06 6.00E-06 8.00E-06 1.00E-05 1.20E-05 1.40E-05 Concentration (M) 30) An extract of rubixanthin of unknown concentration was analyzed with UV-Vis Spectroscopy. The unknown had an absorbance of 0.83. What is the concentration (M) of rubixanthin in the unknown?
A...
(10 pts) The iron content of a well water sample is determined by UV-Vis spectroscopy. A 10.00 mL aliquot of well water is transferred to a 100.0-mL volumetric flask and 20 mL of 0.10 M nitric acid is added to oxidize iron (II) to iron (II). Next 30 mL of a 0.1 M sodium thiocyanate solution is added to form the deep red complex iron (II) thiocyanate. The solution is diluted to volume with DI water. A reagent blank is...
Part 2. UV-Visible Spectroscopy Cut2 concentration Absorbance (mol/L) 0.0010 0.053 0.0025 0.128 0.0050 0.251 0.0075 0.378 0.0100 0.510 Diluted unknown, Trial 1 0.079 For your lab notebook: C. Sketch how you could make each of the the 5 standards for Part 2 from a 0.100 mol/L Cu2+ stock solution, 1.25 mol/L NH3 solution, and Dl water. The procedure is referenced in step 2b. Assume you would use 10-mL volumetric flasks to make the solutions. d. Sketch also how you would...
Table 1. Concentrations of [Fe(Phen),1?, M, in water and UV- vis absorbance values recorded at 2 -505 nm. Concentration of [Fe(Phen)/, M Absorbance 9.500 x 10 0.999 4.250 x 10 0.480 0.219 2.125 10% 1.063 x 10 0.104 0.000 0.003 Table 2. UV-vis absorbance values of [Fe(phen)]* held at 35 °C after mixing with H SO, and recorded at 5 minute intervals (in water at max = 505 nm). Time (minutes) Absorbance 0.997 0.935 0.877 0.823 0.772 0.724 0.680 0.638...
Plot of absorbance vs concentration for calibration curve. Data sheet stapled to back of DRA. Solution Stock solution Dilution 1 (50.0%) Dilution 2 (25.0%) Dilution 3 (12.5%) (SCN) (FeSCNI. 2.0 x10M 2.0 X10M 1.0 x 10-4 M 1.0104M 5.0 x10-5M 5.0*10-5M 2.5 x 10-5Ma.5 x10 -5M 0.836 0.389 0.180 0.085 Calibration curve trendline equation_=43 15.1 % - 0.032 Rvalue of trendline 0.9993 Absorbance Sample Number 0.0020 M Fe(NO), (ml) 0.0020 M KSCN (ML) - - - - - 0. 129...
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