Question

15 pts Due at the beginning of Class: Friday, November 22 lumen into thien is.0362 mm.de of an E.coli soorganisms found in the lumen of our intestines. If E. coli cell is 12.4 mm, and if the concentration of lactose in the hange in free energy of transporting 1 mole of lactose from the 7- 96,500 J/mol-V, R-8.3145 J/mol K. The Assignment 10 75 pts Due at th 10. (A) (3 pts) A) E. coli is one of symbiotic microorganisms found in the lume the concentration of lactose inside of an E. coli cell is 12.4 mM, and if intestinal lumen is .0362 mm, what is the change in free energy of tra lumen into the cytosol of the E. coli cell at 37 909 96.500 J/mol-V, R 8.514. transmembrane electrical potential is -50 mV (Outside-Inside). AG183145J/MVC 310 in- 5J/MV 3101) in 10202 + ( 296500)(-50x10-30) 12.4 (B) (3 pts)The pH of the cytosol of an E. coli cell is 7.48. The is the free energy of transportine 1 mols of Hinto the E. coli cell at 37C7 8.3145 J/mol-K. The transmembrane electrical potential is -50 mV (Outside i cell is 7.48. The pH of the intestinal lumen is 6.62. What into the E. coli cell at 37°C? F-96,500 J/mol V. R 06=(8.3145 J Yank) mor10 Y310k) in + (+) 96500J (-50X60 V) MEN PLSWhat is the free energy of symporting lactose and cytosol of the E. coli cell? from the intestinal lumen into the (D) (3 pts) This is an classical example of what type of transport? coupled transport

Answer 1

00)) Change of bibb's free energy due to tramport om outside to inside of cell A6 = RT en in) +Z FAY ... 0 there, R= universal gan constant = 8.3145 malik I tem Berature at Kelvin - 37° c (physiological tamp) - 27+2 73 x = 300k Win – Concentration of solute inside the cell Concensation of solute out side the cell z - charge of species - fara dar constant =96,500 /mol.n. & ant a conce 44 = membrane Potential differences in change between in of our --Som =-50x10 3 2 -0.05N here, Wina [lactose inside E. coli = 12.4 mm our = [Loctore) ortride B. Colice. in lumen = 0.0362 mm z= 0 for lacton. 46 = 28:3145 x 20 x ln + x 96500 X Coosys = 15042 37 1/mol 15.042 KJ/mol . & Change of free energy for Imol of Lact ore from port in the lumen in 15.042 KI 2.4mm in To 0362 mph)

(10) (3) : PH = -log, tits tram port THtout - log titt in = 7:48 & [147] in = 107.48 - Ci4 t)ün = 3.41x10-8 m Similarly - log Cit to our = 6.62. . [th out = 18-662 2.4 x 10-7M. vising equation () wercan write V Ht into the cell A RT ln [1th & Z FAY here, z = +7 321810-801 A6 = 48.314 Tur 30 xln (2:48187 & 1x 96500 Test mes X (-0.05 Val -- 9999 t/mol – 9.93) KI/mol (An) (0) (c) the free energy change (A. Ktot) for symporting Lectore Abgotas - A A b for tranforting Lectore Total - As for gran porting it to 35. 042)+ (-9-991)3 /10) sim kosmos 10%) this is an example of coupled transport