If 0.500mL of unknown is transfered into a test tube and then a sample is made...
If 0.500mL of unknown is transfered into a test tube and then a sample is made using 0.100 mL of the unknown, 3.00 ml of BCA and 0.900 mL of H2O to make up a total volume of 4.00 ml, what would be the dilution factor?
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If 0.500mL of unknown is transfered into a test tube and then a
sample is made...
I'm a might stuck on some calculations for my lab report on Spectrophotometric Determination of Protein....
I'm a might stuck on some calculations for my lab report on Spectrophotometric Determination of Protein. I'm looking to calculate the concentration of the first standard. Sample Vol. of Protein Std Solution Vol. of BCA reagent Volumeof DI water Total Vol. Vol of Unknown Blank 0 3.00 1.000 4.00 0 Standard 1 0.100 3.00 0.900 4.00 0 Standard 2 0.200 3.00 0.800 4.00 0 Standard 3 0.300 3.00 0.700 4.00 0 Standard 4 0.400 3.00 0.600 4.00 0 Standard 5...
I am stuck on how to find concentration for standards and unknowns. Any kind of help would be appreciated. The correct...
I am stuck on how to find concentration for standards and
unknowns. Any kind of help would be appreciated.
The corrected absorbance is = Blank - absorbance
1. Prepare the BCA working reagent. Use a graduated cylinder and add 30 ml of the BCA reagent into a 50 ml Erlenmeyer flask. Use the appropriate micropipette to add 600 uL of the CuSO4 solution into the flask and mix by swirling the flask. Record the exact mass of albumin and the...
1) if 5 parts sample is added to 10 parts diluents, what is the dilution that...
1) if 5 parts sample is added to 10 parts diluents, what is the dilution that was just made? 2. What is the ratio if 5 parts sample volume is added to 10 parts diluents volume? 3. In a 1 to 3 dilution, what is the dilution FACTOR? 4. In a 1 to 10 dilution, what is the dilution FACTOR? 5. What is the dilution FACTOR if 40 mcL of sample added to 260 mcL of diluent? 6. What is...
Answer and do the following calculations: 1. Suppose your professor handed you a test tube with...
Answer and do the following calculations: 1. Suppose your professor handed you a test tube with 2.0 mL of an E. coli broth culture in it and told you to make a 10–2 dilution of the entire culture. Explain how you would do this. Show your calculations. 2. How would you produce a 10–1 dilution of a 3 mL bacterial sample using the entire 3 mL volume? 3. You have 0.05 mL of an undiluted culture at a concentration of...
3. A serial dilution of a swab sample was prepared by adding 10 uL to a...
3. A serial dilution of a swab sample was prepared by adding 10 uL to a volume of 990 ML water in tube 1, and 10 uL to a volume of 990 uL water in tube 2. a. What is the overall dilution and dilution factor of each tube? Overall Dilution Dilution Factor Tube 1 Tube 2 b. If 100 uL of tube 2 is spread plated and 126 colonies grow, what was the #cells/mL in the original sample?
6. Suppose your TA gives you a test tube containing a phosphate solution of unknown concentration...
6. Suppose your TA gives you a test tube containing a phosphate solution of unknown concentration and asks you to analyze it. Your TA is kind enough to give you a hint: the unknown solution has a phosphate concentration between 4.00 x 105 M and 8.00 x 103M. How would your sample preparation procedure for analyzing this unknown solution differ from the sample preparation procedure for the soda pop?
6. Suppose your TA gives you a test tube containing a...
.Viable plate count Using the formula: cfu/ml = (no. of colonies x dilution factor) / volume...
.Viable plate count Using the formula: cfu/ml = (no. of colonies x dilution factor) / volume of culture plate In plate 10-4 there were 150 colonies counted. Experimental process: Pipet 0.25 mL of the S. cerevisiae solution into the test tube labeled 10-1. Pipet the solution up and down several times to ensure all organisms are rinsed from the pipet into the test tube. Pipet 0.25 mL of the 10-1 S. cerevisiae solution into the test tube labeled 10-2. Pipet...
A student made solution #3 using the experimental method in this lab, and measured an absorbance of 0.559. The starting...
A student made solution #3 using the experimental method in this
lab, and measured an absorbance of 0.559. The starting reagents are
2.00 x 10-3 M Fe(NO3)3 and 2.00 x
10-3 M KSCN.
The amount of absorption is proportional to the concentration
of
FeSCN2+. This relationship – true for many solutions – is called
“Beer’s Law”, and has the simple equation:
A = bc
where “A” is the absorption, “b” is 5174.6 for FeSCN2+ and “c”
is molarity
Make Five...
We first made a 1.0 Mcfarland inoculum with a 10ml volume from a few isolated colonies....
We first made a 1.0 Mcfarland inoculum with a 10ml volume from a few isolated colonies. Then we did the following dilutions: 10^6 dilution, add 0.1 mL of the Mcfarland inoculum to test tube #2 and add 10ml of water and mix. 10^4 dilution, add 0.1 mL of test tube #2 to test tube #3 and add 10ml of water and mix. 10^2 dilution, , add 0.1 mL of test tube #3 to test tube #4 and add 10ml of...
1)How many microliters of original sample are required to produce a final dilution of 10-2 in...
1)How many microliters of original sample are required to produce a final dilution of 10-2 in a total volume of 5 mL? 1 microliter is 10-6 L or 10-3 mL. 2)A 0.00001 dilution is performed on a culture of bacteria in order to perform viable plate counts. From the dilution, *0.1 mL* of solution is plated on solid media, and 197 colony forming units grow on the plate. How many bacteria are in a single mL of the original culture?...
I am stuck on how to find concentration for standards and
unknowns. Any kind of help would be appreciated.
The corrected absorbance is = Blank - absorbance
1. Prepare the BCA working reagent. Use a graduated cylinder and add 30 ml of the BCA reagent into a 50 ml Erlenmeyer flask. Use the appropriate micropipette to add 600 uL of the CuSO4 solution into the flask and mix by swirling the flask. Record the exact mass of albumin and the...
3. A serial dilution of a swab sample was prepared by adding 10 uL to a volume of 990 ML water in tube 1, and 10 uL to a volume of 990 uL water in tube 2. a. What is the overall dilution and dilution factor of each tube? Overall Dilution Dilution Factor Tube 1 Tube 2 b. If 100 uL of tube 2 is spread plated and 126 colonies grow, what was the #cells/mL in the original sample?
6. Suppose your TA gives you a test tube containing a phosphate solution of unknown concentration and asks you to analyze it. Your TA is kind enough to give you a hint: the unknown solution has a phosphate concentration between 4.00 x 105 M and 8.00 x 103M. How would your sample preparation procedure for analyzing this unknown solution differ from the sample preparation procedure for the soda pop?
6. Suppose your TA gives you a test tube containing a...
A student made solution #3 using the experimental method in this
lab, and measured an absorbance of 0.559. The starting reagents are
2.00 x 10-3 M Fe(NO3)3 and 2.00 x
10-3 M KSCN.
The amount of absorption is proportional to the concentration
of
FeSCN2+. This relationship – true for many solutions – is called
“Beer’s Law”, and has the simple equation:
A = bc
where “A” is the absorption, “b” is 5174.6 for FeSCN2+ and “c”
is molarity
Make Five...
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