In TLC, Why were the solvents concentrations changed during procedures?
In TLC, Why were the solvents concentrations changed during procedures?
Homework Answers
Change in solvent concentration indirectly affect polarity of solvent. For example , Benzene being a non - polar solvent , during dilution with water , it's concentration decrease but polarity Increases due to addition of non polar H2O.
Low polarity compound elute with low polarity solvent where as high polarity compound elute with high polarity solvent .
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In TLC, Why were the solvents concentrations changed during
procedures?
Common solvents used in TLC analysis are hexane and ethyl acetate, the latter being more polar. A TLC has been performed...
Common solvents used in TLC analysis are hexane and ethyl acetate, the latter being more polar. A TLC has been performed on a mixture of two compounds. The solvent ran for 3 cm(plate length is 6cm) with 5% ethyl acetate in hexane. The two spots were not resolved, meaning they showed up too close to each other. What can you suggest to possible improve the results of the separation?
1. Each of the materials and solvents listed below were waste generated during an experiment. Place...
1. Each of the materials and solvents listed below were waste generated during an experiment. Place each waste listed in the correct column. (i) used hexane (ii), used dichloromethane (iii), filter paper with used CaCl2 (iv), used silica from column chromatography (v), broken glass pipette (vi), used TLC plate (vii), chloroform (viii), used urea (ix), used filter paper (x), broken capillary tubes Laboratory Sink | Halogenated Waste drum Non-Halogenated Glass waste box Waste drum solid waste Container
1) Why do we use a mixture of ethyl acetate and hexanes as solvents in the...
1) Why do we use a mixture of ethyl acetate and hexanes as solvents in the synthesis of 5,6-endo-dicarboxylic anhydride? 2) What do the terms exo and endo refer to? 3) Why do we not do any TLC on the product in the synthesis of 5,6-endo-dicarboxylic anhydride?
3) What two methods of visualizing spots on the TLC plate will you use in this lab? Why would you predict that the...
3) What two methods of visualizing spots on the TLC plate will you use in this lab? Why would you predict that the elution solvents (hexanes or ethyl acetate) would not be visible under UV visualization?
Why ink must be avoded in marking TLC plates? Why is TLC analysis limited to nonvolatile...
Why ink must be avoded in marking TLC plates? Why is TLC analysis limited to nonvolatile samples?
TLC Analysis Of Egg Lipids Lab 1. The TLC plates were pre-baked at >120C. Why is...
TLC Analysis Of Egg Lipids Lab 1. The TLC plates were pre-baked at >120C. Why is this step necessary? 2. Below is the structure of your lipid staining dye, Naphthol Blue-Black. Explain which noncovalent interactions would be most important in allowing the dye to adhere to lipids (consider the chemical property of lipids as well as the dye). 3. You ran a solution of a fatty acid and a wax on a TLC plate using the exact conditions used in...
Why were solvents used to extract the pigments from spinach? We used 3 mL of acetone...
Why were solvents used to extract the pigments from spinach? We used 3 mL of acetone and 3 mL of hexane and then ground the spinach leave in a mortar for 5 minutes.
What is the main purpose of running a column chromatography? Similar to TLC. column chromatography is...
What is the main purpose of running a column chromatography? Similar to TLC. column chromatography is based on analytes being partitioned between a stationary phase (not moving) and a mobile phase (moving). What is most commonly used as stationary phase in a column? Is this material polar or non-polar? In this experiment, you will use different solvents to run column chromatography separation of different pigments extracted from spinach leaves. These solvents are a mixture of acetone and hexanes. (a) What...
1. Why should any marks on the TLC y marks on the TLC plate be done...
1. Why should any marks on the TLC y marks on the TLC plate be done in pencil and not pen? 2. A spot on a TLC plat migrates 4.2 cm as the solvent plates my migrates 4.2 cm as the solvent plates migrates 8.0 cm up the plate. What is the Rivalue for this spot? Show your calculati
When observing the TLC plates, were all the spots nice small circles? If no, provide and...
When observing the TLC plates, were all the spots nice small circles? If no, provide and explanation as to why this might be the case. 3.
1. Each of the materials and solvents listed below were waste generated during an experiment. Place each waste listed in the correct column. (i) used hexane (ii), used dichloromethane (iii), filter paper with used CaCl2 (iv), used silica from column chromatography (v), broken glass pipette (vi), used TLC plate (vii), chloroform (viii), used urea (ix), used filter paper (x), broken capillary tubes Laboratory Sink | Halogenated Waste drum Non-Halogenated Glass waste box Waste drum solid waste Container
3) What two methods of visualizing spots on the TLC plate will you use in this lab? Why would you predict that the elution solvents (hexanes or ethyl acetate) would not be visible under UV visualization?
What is the main purpose of running a column chromatography? Similar to TLC. column chromatography is based on analytes being partitioned between a stationary phase (not moving) and a mobile phase (moving). What is most commonly used as stationary phase in a column? Is this material polar or non-polar? In this experiment, you will use different solvents to run column chromatography separation of different pigments extracted from spinach leaves. These solvents are a mixture of acetone and hexanes. (a) What...
1. Why should any marks on the TLC y marks on the TLC plate be done in pencil and not pen? 2. A spot on a TLC plat migrates 4.2 cm as the solvent plates my migrates 4.2 cm as the solvent plates migrates 8.0 cm up the plate. What is the Rivalue for this spot? Show your calculati
When observing the TLC plates, were all the spots nice small circles? If no, provide and explanation as to why this might be the case. 3.
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