Why were solvents used to extract the pigments from spinach? We used 3 mL of acetone...
Why were solvents used to extract the pigments from spinach?
We used 3 mL of acetone and 3 mL of hexane and then ground the spinach leave in a mortar for 5 minutes.
Homework Answers
Generally, organic solvents such as Acetone, dichloromethane, ethylacetate, ethanol can be used for extraction of compounds The pigments from spinach are organic compounds. Organic compounds are better soluble in organic solvents. Hence we we grind spinach and add solvent the pigments are soluble in the solvent. Then we can take solvent and concentrate. These organic solvent having less boiling point. So that we can concentrate and isolate the pigments very easily. Due to these reasons, the solvent particularly easily volatile solvents are used.
Add Answer to:
Why were solvents used to extract the pigments from spinach?
We used 3 mL of acetone...
What happened to the spinach protein? Which layer would the proteins be in? Background needed: We...
What happened to the spinach protein? Which layer would the proteins be in? Background needed: We ground up a spinach leaf in a mortar for 5 minutes with 3 mL of acetone and 3 mL of hexane. We then filtered the green solution (avoiding the ground leaves) into a filtering pipet. 2 mL of saturated aqueous sodium chloride were added to the test tube with the filtered solution in order to make **two layers**. The top, more green layer was...
Pre lab Introduction Your instructor has prepared an extract of chloroplast pigments from fresh green grass...
Pre lab
Introduction Your instructor has prepared an extract of chloroplast pigments from fresh green grass or fresh spinach. A blender was used to rupture the cells, and the pigments were then extracted with acetone, an organic solvent. Work- ing with one other student, begin this exercise by separating the pigments extracted using paper chromatography. To do this, you will apply the pig- ment extract to a cylinder of chromatographic paper. You will then place the ylinder in a jar...
please do two pargraph about this pre lab and lab include Title,purpose ,chemical eqution if their...
please do two pargraph about this pre lab and lab include
Title,purpose ,chemical eqution if their any ،Procedure
,Hazards(hazard Identification,physical and chemical
properties),Experimental Phase, Data
&Observations,Data&Observations cont (melting point and
meling point). and Calcuations
Spinach Laboratory Procedure PROCEDURES Isolation of pigment from leaves: 1. Wash the spinach leaves. Weigh 1.0 g-2.0 g of dry spinach leaves. Remove any stems and veins from the leaves before you weigh. 2. Combine 1.0 g of anhydrous magnesium sulfate with 0.5 g of sand...
-Give a brief background on the role of pigments in photosythensis. -state your hypothesis. -finish with...
-Give a brief background on the role of pigments in
photosythensis.
-state your hypothesis.
-finish with stating the rationale for your hypothesis (how
did you arrive at these hypothesis?)
this is an introduction piece, so it doesn't need the results to
write the papee. introduction sections set up the background,
purpose, rationale, and hypothesis for a study.
Biod 20 PHOTOSYNTHESIS Background Plants harness the energy of the sun through a process called photosynthesis. The leaf is the plant organ that...
Objective To extract essential oils and other natural products from the leaves of mentha spicata L....
Objective To extract essential oils and other natural products from the leaves of mentha spicata L. . Reactions Not applicable Procedure 1. Weigh 1.0 g of leaves and place them in a glass mortar. Grind the material until a more or less homogenous mass is obtained. Transfer the ground material into a 100 mL beaker, add a stir bar and 10 mL of water and 10 mL of diethyl ether. Cover the beaker with a watch glass and stir vigorously for 10 minutes...
1. Identify and explain which solvents can be used from the list below to perform the...
1. Identify and explain which solvents can be used from the list below to perform the extraction described in this experiment. Ethanol Acetone Diethyl ether Methylene chloride Benzene Toluene Methanol Carbon tetrachloride Acetic acid 2-propanol 2. An aqueous solution of a dye with molar extinction coefficient of 60,000, has an absorbance of 0.8 when a 1 cm cuvette is used. Calculate the concentration of the dye using Beer’s Law. (MW of the dye = 374) 3. A 20 mL portion...
Please show any work that you can!! This is all the info that is given, What...
Please show any work that you can!!
This is all the info that is given, What info is missing??
Problem #10: (20 points) 7. A student separated the pigments from spinach extract using column chromatography. The B-carotene fraction was 10 mL, but when the student measured the absorption at 455 nm, it was greater than one so the student decided to dilute the sample. The sample was diluted by taking 1 mL of the B-carotene fraction and diluting it to...
These questions are from an experiment where we had a mixture of Ferrocene, acetylferrocene and diacetyl...
These questions are from an experiment where we had a mixture of Ferrocene, acetylferrocene and diacetyl ferrocene and we separated each by using column chromatography. Before doing the column chromatography we did TLC analysis to ensure that we did, in fact, have a mixture of these 3 compounds. During the column chromatography we first eluted the ferrocene using pure hexane. Then we eluted the acetylferrocene using a 1:1 mixture of hexane and methylene chloride. Then we eluted the diacetylferrocene using...
plz help complete my prelab heres the first page 3. A third reaction mixture was made up in the following way: 5 mL. 4.0 M acetone+ 5 mL 0.0050 M12+ 10 mL 1.0M HCI+5 mL H2O a. What were the initi...
plz help complete my prelab
heres the first page
3. A third reaction mixture was made up in the following way: 5 mL. 4.0 M acetone+ 5 mL 0.0050 M12+ 10 mL 1.0M HCI+5 mL H2O a. What were the initial concentrations of acetone, 12 and H' ion in the reaction mixture? b. It took 109 seconds for the 12 color to disappear from the reaction mixture when at the same temperature as in question 1. What was the rate...
#3 An absorber is to be used to remove acetone from air entering gas stream is...
#3 An absorber is to be used to remove acetone from air entering gas stream is 2 mole % acetone and enters at a rate of 40 kmol/hr. The water enters at a rate of 120 kmol hr. Both streams enter at 293 K and 1.4 atm pressure. We wish to remove 90 % of the entering acetone in this column. a. What is the minimum water flow to accomplish this separation? b. How many equilibrium stages would be required...
Pre lab
Introduction Your instructor has prepared an extract of chloroplast pigments from fresh green grass or fresh spinach. A blender was used to rupture the cells, and the pigments were then extracted with acetone, an organic solvent. Work- ing with one other student, begin this exercise by separating the pigments extracted using paper chromatography. To do this, you will apply the pig- ment extract to a cylinder of chromatographic paper. You will then place the ylinder in a jar...
please do two pargraph about this pre lab and lab include
Title,purpose ,chemical eqution if their any ،Procedure
,Hazards(hazard Identification,physical and chemical
properties),Experimental Phase, Data
&Observations,Data&Observations cont (melting point and
meling point). and Calcuations
Spinach Laboratory Procedure PROCEDURES Isolation of pigment from leaves: 1. Wash the spinach leaves. Weigh 1.0 g-2.0 g of dry spinach leaves. Remove any stems and veins from the leaves before you weigh. 2. Combine 1.0 g of anhydrous magnesium sulfate with 0.5 g of sand...
-Give a brief background on the role of pigments in
photosythensis.
-state your hypothesis.
-finish with stating the rationale for your hypothesis (how
did you arrive at these hypothesis?)
this is an introduction piece, so it doesn't need the results to
write the papee. introduction sections set up the background,
purpose, rationale, and hypothesis for a study.
Biod 20 PHOTOSYNTHESIS Background Plants harness the energy of the sun through a process called photosynthesis. The leaf is the plant organ that...
Please show any work that you can!!
This is all the info that is given, What info is missing??
Problem #10: (20 points) 7. A student separated the pigments from spinach extract using column chromatography. The B-carotene fraction was 10 mL, but when the student measured the absorption at 455 nm, it was greater than one so the student decided to dilute the sample. The sample was diluted by taking 1 mL of the B-carotene fraction and diluting it to...
plz help complete my prelab
heres the first page
3. A third reaction mixture was made up in the following way: 5 mL. 4.0 M acetone+ 5 mL 0.0050 M12+ 10 mL 1.0M HCI+5 mL H2O a. What were the initial concentrations of acetone, 12 and H' ion in the reaction mixture? b. It took 109 seconds for the 12 color to disappear from the reaction mixture when at the same temperature as in question 1. What was the rate...
#3 An absorber is to be used to remove acetone from air entering gas stream is 2 mole % acetone and enters at a rate of 40 kmol/hr. The water enters at a rate of 120 kmol hr. Both streams enter at 293 K and 1.4 atm pressure. We wish to remove 90 % of the entering acetone in this column. a. What is the minimum water flow to accomplish this separation? b. How many equilibrium stages would be required...
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