1. e. In transmission electron microscopy , the sample is taken to form an image where the image is formed by transmission of electrons through the specimen or sample. The specimen or sample will be very thin section which is less than 100nm in thickness or the suspension over the grid.
2. E. In my opinion the answer might be transmission electron microscopy because these are used with the help of beam of lights which is capable of increasing the resolution of the image over light microscope. The whole bacteria or the object is coated with gold or palladium which are metals in order to view the 3- D image of the bacteria which is done by creating dark or light images of the organism on the monitor.
3. E. Transmission electron microscopy or TEM is a technique of microscopy in which the beam or the light of the electrons are communicated or transfered through a sample in order to form a proper image .
4. D. Scanning electron microscopy is used to scan the surface of the microorganism because it helps in production of the image of the sample with the help of focused beam electrons. This is done by interaction of electrons with the sample's atoms present.
5.a. The gram positive bacteria have the cell wall which is very thick in texture which will make them to absorb all the stains which makes them turn into purple colour because of the absorption of the crystal violets which are used as dyes.
Microblology 208 Summer 2019 Exam 2 1) A thin section of a bacterial Sample is cut...
Match the information provided to the microscopy technique best suited for it. Both transmission and reflection techniques are possible. Operates on quantum mechanical principles. Can operate in contact with the sample. A fluorescent screen is needed to make up or record an image. Both surface topography and chemistry play a role in the final image. A. Optical microscopy B. Scanning electron microscopy (SEM) C. Scanning tunneling microscopy (STM) D. Atomic force microscopy (AFM) E. Tunneling electron microscope (TEM)
121. A graduate student is studying a pathological bacterial strain. As a part of a project to develop an antibiotic against the bacterial strain, the student needs to determine the number of proteins present in the ribosome. The best technique for investigating this question is: A. Cell homogenization and centrifugation B. Phase contrast microscopy C. Brightfield microscopy D. Scanning electron microscopy E. Transmission electron microscopy
Tabulate the similarities and differences between TEM(Transmission electron microscopy) and SEM(Scanning Electron Microscopy) regarding (a) sample preparation (4), (b) processing (4), (c) instrumentation (4), (d) detection (4) and (e) the structures that can be observed (3), (f) staining (3), (g) IHC (4) and (h) ISH (4).
2)Which of the A)peptidoglycan B)cellulose C)glycogen D)chitin 3 Which of the following is true of fluorescence microscopy? A) Fluorescence microscopy is best at viewing rounded, thicker specimens B) Fluorescent light is emitted throughout the specimen due to fluorescent dyes conjugated to antibodies. C) Fluorescence microscopy is used to view dead specimens only. D) Fluorescence microscopy presents images in three dimensions. E) Fluorescence microscopy is able to overcome problems encountered with using confocal scanning microscopy. 4)While synthesizing a new green pigment,...
B. The plasma memibrane buds off exosomes from the surface C. They come from the nucleus theough the D. Specialized vesicles from E. Late endosomes fuse from the surface mitochondria bud off and are secreted with the plasma membrane and release their content is a minus end directed motor on microtubules A. Dynein B. Myosin C. Kinesin D. Motorin .E. All of the above 19. M ovement of molecules from one side of an epithelial Iayer so the oher is...
Please answer the questions 12 to 21 shown in the photo
attachment accurately. Please. Thank you!
12. The following is incorrect regarding mitochondria and plastids: A) They originated by invagination rial DNA is homologous to that of ricketsias C) Chloroplasts originated from D) They have bacterial-type 70S ribosomes E) None of the above. 13. One of these techniques is mismatched with the specimen under study. Which one is i? on bacterial cell wall surfaces-Atomi force microscopy B) Stained bacterial cells-Bright-field...
1. Fill out the following table by indicating which general technique (light microscopy (LM) or electron microscopy (EM]) could be used to observe each structure or phenomenon. Put "no" in the box if the technique could not be used. If light microscopy can be used, name one technique (bright-field, phase-contrast, fluorescence, etc.) that you think would be effective. You will find some useful information in Appendix 1 of this manual and Chapter 18 of your textbook. Structure or phenomenon Could...
1) How are River's postulates for viruses different than Koch's postulates for bacteria? (4 points) 2) For the following tests, indicate what the test is measuring or identifying: (7 points) a. Electron microscopy b. Immunofluorescence microscopy c. ELISA d. Hemagglutinin assays e. Culture f. PCR g. DNA microarray 3) A new disease is circulating among St. Cloud State University. The patient's symptoms are a high fever, runny nose, and cough. How would you use any of the diagnostic tests in...
MATCHING: 4. Molecular biological tools and methodologies are being developed at a blinding pace. Choosing the right tool. therefore. becomes a formidable yet critical task for the successful research seientist. From the following partial list, choose the BEST or MOST APPROPRIATE tool or technique for each of the problems or experiments described Not all |Answers must not be used more than once. answers will be used. 2 points each] A. Autoradiography B. Edman degradation C. FACS (luorescence-activated cell-sorting) D. Hybridoma...
1.Vp and Vg are the phase velocity and group velocity, respectively, of the de Broglie wave for an electron moving with the velocity V, and C is the velocity of light in free space. Which of the following is incorrect? (a) Vp> Vg (b) Vp>C (c) Vg<C (d) Vg=V (e) Vp=V 2.An atomic force microscope (AFM) probe is essentially a cantilever with a nanosized tip at the free end. One scan mode of AFM is to drive the probe as...