Question

1 Which of the following represents one of the mechanisms by which antisense agents exert their effect? a. A short DNA sequence binds to specific regions of complementary mRNA, inducing a nuclease that promotes translation to the corresponding protein b. Antisense oligonucleotides prevent the natural silencing of targeted mRNA sequences C. A short DNA sequence binds to specific regions of complementary mRNA, inducing a nuclease that cleaves the mRNA d. Antisense oligonucleotides inhibit all types of gene splicing 2 The siRNAs are usually a. 7-8 bp long b. 11-13 bp long C. 20-21 bp long d. 50-60 bp long 3 Which of the following are the main considerations for design of an antisense therapeutic agent a. Delivery to the site of action in the body without the drug being degraded by nucleases b. Entry into the cell of the target tissue and colocalize with its target RNA at a sufficient concentration for a bimolecular reaction to occur C. Structure that favors association with the target RNA and be designed to bind to a RNA region that is vulnerable to binding d. All of the above

Answer 1

1. d. Antisense oligonucleotides inhibits all types of gene splicing.

Antisense mechanism is used for inhibition of gene expression.The antisense nucleic acid pairs with complementary RNA,preventing it from being translated into a protein.

2. c.20-21 bp long

3. d. All of the above.